@inbook{93f5b2c547e34d028d443dea6402d896,
title = "Understanding Microtubule Organizing Centers by Comparing Mutant and Wild-Type Structures with Electron Tomography",
abstract = "The unicellular alga, Chlamydomonas, is a useful model system in which to study MTOCs because of the powerful genetic and molecular techniques available for this organism. Recent advances in specimen preparation that involve rapid freezing followed by freeze-substitution result in close-to-optimal preservation of the basal body complex. Coupling genetics with 3D structure studies of optimally preserved cells further enhances our understanding of basal body assembly and function. Finally, these methods can be applied to a wide range of biological systems to address structure-function relationships in other organisms.",
author = "O'Toole, {Eileen T.} and Giddings, {Thomas H.} and Dutcher, {Susan K.}",
note = "Funding Information: We thank David Mastronarde for developing the automated image capture software and for helpful advice on tomographic reconstruction. We thank Andrew Staehelin for use of the high‐pressure freezer and Mary Morphew for useful suggestions for specimen preparation. This work was supported by Grant RR‐000592 from the National Center for Research Resources of the National Institutes of Health to J. R. McIntosh.",
year = "2007",
doi = "10.1016/S0091-679X(06)79005-7",
language = "English",
isbn = "0123706475",
series = "Methods in Cell Biology",
number = "79",
pages = "125--143",
editor = "J.R. McIntosh",
booktitle = "Cellular Electron Microscopy",
edition = "79",
}