Understanding Microtubule Organizing Centers by Comparing Mutant and Wild-Type Structures with Electron Tomography

Eileen T. O'Toole, Thomas H. Giddings, Susan K. Dutcher

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

30 Scopus citations

Abstract

The unicellular alga, Chlamydomonas, is a useful model system in which to study MTOCs because of the powerful genetic and molecular techniques available for this organism. Recent advances in specimen preparation that involve rapid freezing followed by freeze-substitution result in close-to-optimal preservation of the basal body complex. Coupling genetics with 3D structure studies of optimally preserved cells further enhances our understanding of basal body assembly and function. Finally, these methods can be applied to a wide range of biological systems to address structure-function relationships in other organisms.

Original languageEnglish
Title of host publicationCellular Electron Microscopy
EditorsJ.R. McIntosh
Pages125-143
Number of pages19
Edition79
DOIs
StatePublished - Feb 28 2007

Publication series

NameMethods in Cell Biology
Number79
Volume2007
ISSN (Print)0091-679X

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