@article{446c30e12d6e45c3b358577b13e82715,
title = "Unc5C and DCC act downstream of Ctip2 and Satb2 and contribute to corpus callosum formation",
abstract = "The pyramidal neurons of the mammalian neocortex form two major types of long-range connections-corticocortical and cortico-subcortical. The transcription factors Satb2 and Ctip2 are critical regulators of neuronal cell fate that control interhemispheric versus corticofugal connections respectively. Here, we investigate the axon guidance molecules downstream of Satb2 and Ctip2 that establish these connections. We show that the expression of two Netrin1 receptors-DCC and Unc5C is under direct negative regulation by Satb2 and Ctip2, respectively. Further, we show that the Netrin1-Unc5C/DCC interaction is involved in controlling the interhemispherical projection in a subset of early born, deep layer callosal neurons.",
author = "Swathi Srivatsa and Srinivas Parthasarathy and Olga Britanova and Ingo Bormuth and Donahoo, {Amber Lee} and Ackerman, {Susan L.} and Richards, {Linda J.} and Victor Tarabykin",
note = "Funding Information: We would like to thank the following for generously sharing their materials and resources-Dr Kominami for the Ctip2-mutant mice, Dr Helen Cooper for the DCC antibody, Dr Franck Polleux for the CAG-Unc5C expression plasmid, Dr Handjanto-nakis for the CAG Venus-GFP plasmid, Dr Leid for providing the Ctip2 expression plasmid used in luciferase assays, Dr Nenad Sestan for the Satb2 overexpression plasmid and Dr Suzana Atanasoski for the pCAG-Ski plasmid. SP would like to thank Dr Zoltan Molnar for teaching the art of DiI tracing. The authors would also like to acknowledge the following for their contribution towards this work—Roman Wunderlich and Manuela Schwark for technical help, Paraskevi Sgourdou (former member V.T. lab) for pilot experiments and discussion and Steffen Schuster for help with western blot analysis. This work was supported by SFB665, DFG Heisenberg programme, DFG grant TA 303/3, Exzellenzcluster 257, National Health and Medical Research Council Australia, Project Grant 1043045 (L.J.R.), Max-Planck Society Fritz Thysson Foundation, Boehringer Ingelheim Fonds PhD Fellowships (S.S. and S.P.), Australian postgraduate award and Queensland Brain Institute top-up award (A-L.SD). L.J.R. is supported by a Principal Research Fellowship from the National health and Medical Research Council, Australia.",
year = "2014",
month = apr,
day = "17",
doi = "10.1038/ncomms4708",
language = "English",
volume = "5",
journal = "Nature communications",
issn = "2041-1723",
}