Ultrasound-induced cell membrane porosity

Cheri X. Deng; Fred Sieling; Hua Pan; Jianmin Cui

doi:10.1016/j.ultrasmedbio.2004.01.005

Ultrasound-induced cell membrane porosity

Cheri X. Deng, Fred Sieling, Hua Pan, Jianmin Cui

Research output: Contribution to journal › Article › peer-review

315 Scopus citations

Abstract

Recent studies of ultrasound (US) methods for targeted drug delivery and nonviral gene transfection revealed new, advantageous possibilities. These studies utilized US contrast agents, commonly stabilized microbubbles, to facilitate delivery and suggested that US delivery resulted from cell sonoporation, the formation of temporary pores in the cell membrane induced by US. Using voltage clamp techniques, we obtained real-time measurements of sonoporation of single Xenopus oocyte in the presence of Optison™, an agent consisting of albumin-shelled C₃F₈ gas bubbles (mean diameter 3.2 μm). Ultrasound increased the transmembrane current as a direct result of decreased membrane resistance due to pore formation. We observed a distinct delay of sonoporation following US activation and characteristic stepwise increases of transmembrane current throughout US duration. We discovered that the resealing of cell membrane following US exposure required Ca²⁺ entering the cell through US-induced pores.

Original language	English
Pages (from-to)	519-526
Number of pages	8
Journal	Ultrasound in Medicine and Biology
Volume	30
Issue number	4
DOIs	https://doi.org/10.1016/j.ultrasmedbio.2004.01.005
State	Published - Apr 2004

Keywords

Cell membrane
Drug delivery
Gene delivery
Sonoporation
Ultrasound contrast agent

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10.1016/j.ultrasmedbio.2004.01.005

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title = "Ultrasound-induced cell membrane porosity",

abstract = "Recent studies of ultrasound (US) methods for targeted drug delivery and nonviral gene transfection revealed new, advantageous possibilities. These studies utilized US contrast agents, commonly stabilized microbubbles, to facilitate delivery and suggested that US delivery resulted from cell sonoporation, the formation of temporary pores in the cell membrane induced by US. Using voltage clamp techniques, we obtained real-time measurements of sonoporation of single Xenopus oocyte in the presence of Optison{\texttrademark}, an agent consisting of albumin-shelled C3F8 gas bubbles (mean diameter 3.2 μm). Ultrasound increased the transmembrane current as a direct result of decreased membrane resistance due to pore formation. We observed a distinct delay of sonoporation following US activation and characteristic stepwise increases of transmembrane current throughout US duration. We discovered that the resealing of cell membrane following US exposure required Ca2+ entering the cell through US-induced pores.",

keywords = "Cell membrane, Drug delivery, Gene delivery, Sonoporation, Ultrasound contrast agent",

author = "Deng, {Cheri X.} and Fred Sieling and Hua Pan and Jianmin Cui",

note = "Funding Information: This work was supported by grants from the NIH (HL70393) and the Whitaker Foundation (RG 00 to 0396) (to J. Cui) and start-up funding (to C. X. Deng) from the Department of Biomedical Engineering, Case Western Reserve University. ",

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AU - Deng, Cheri X.

AU - Sieling, Fred

AU - Pan, Hua

AU - Cui, Jianmin

N1 - Funding Information: This work was supported by grants from the NIH (HL70393) and the Whitaker Foundation (RG 00 to 0396) (to J. Cui) and start-up funding (to C. X. Deng) from the Department of Biomedical Engineering, Case Western Reserve University.

PY - 2004/4

Y1 - 2004/4

N2 - Recent studies of ultrasound (US) methods for targeted drug delivery and nonviral gene transfection revealed new, advantageous possibilities. These studies utilized US contrast agents, commonly stabilized microbubbles, to facilitate delivery and suggested that US delivery resulted from cell sonoporation, the formation of temporary pores in the cell membrane induced by US. Using voltage clamp techniques, we obtained real-time measurements of sonoporation of single Xenopus oocyte in the presence of Optison™, an agent consisting of albumin-shelled C3F8 gas bubbles (mean diameter 3.2 μm). Ultrasound increased the transmembrane current as a direct result of decreased membrane resistance due to pore formation. We observed a distinct delay of sonoporation following US activation and characteristic stepwise increases of transmembrane current throughout US duration. We discovered that the resealing of cell membrane following US exposure required Ca2+ entering the cell through US-induced pores.

AB - Recent studies of ultrasound (US) methods for targeted drug delivery and nonviral gene transfection revealed new, advantageous possibilities. These studies utilized US contrast agents, commonly stabilized microbubbles, to facilitate delivery and suggested that US delivery resulted from cell sonoporation, the formation of temporary pores in the cell membrane induced by US. Using voltage clamp techniques, we obtained real-time measurements of sonoporation of single Xenopus oocyte in the presence of Optison™, an agent consisting of albumin-shelled C3F8 gas bubbles (mean diameter 3.2 μm). Ultrasound increased the transmembrane current as a direct result of decreased membrane resistance due to pore formation. We observed a distinct delay of sonoporation following US activation and characteristic stepwise increases of transmembrane current throughout US duration. We discovered that the resealing of cell membrane following US exposure required Ca2+ entering the cell through US-induced pores.

KW - Cell membrane

KW - Drug delivery

KW - Gene delivery

KW - Sonoporation

KW - Ultrasound contrast agent

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DO - 10.1016/j.ultrasmedbio.2004.01.005

M3 - Article

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AN - SCOPUS:2342512231

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VL - 30

SP - 519

EP - 526

JO - Ultrasound in Medicine and Biology

JF - Ultrasound in Medicine and Biology

IS - 4

ER -

Ultrasound-induced cell membrane porosity

Abstract

Keywords

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