Ultra-low frequency labeling of single cell lineages in Drosophila

Mark Snee, Wen Chih Lee, Ryan Beehler-Evans, Craig A. Micchelli

Research output: Contribution to journalArticlepeer-review


We describe a new methodology for genetically labeling single cell lineages in Drosophila called DMARCM. The system offers ultra-low frequency labeling, linear induction, consistent labeling among individuals and virtually no background signal. We compare this technique to an existing approach, which has been widely adopted. We demonstrate how application of DMARCM in the gastrointestinal epithelium permits the effects of labeling frequency on tumorigenic stem cell growth to be distinguished in an established tumor model.

Original languageEnglish
Pages (from-to)9-12
Number of pages4
JournalDevelopmental Biology
Issue number1
StatePublished - Jan 1 2020


  • Cell lineage analysis
  • Drosophila
  • Monoclonality
  • Stem cells
  • Tumorigenesis


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