Ultra-low frequency labeling of single cell lineages in Drosophila

Mark Snee; Wen Chih Lee; Ryan Beehler-Evans; Craig A. Micchelli

doi:10.1016/j.ydbio.2019.09.008

Ultra-low frequency labeling of single cell lineages in Drosophila

Mark Snee, Wen Chih Lee, Ryan Beehler-Evans, Craig A. Micchelli

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a new methodology for genetically labeling single cell lineages in Drosophila called DMARCM. The system offers ultra-low frequency labeling, linear induction, consistent labeling among individuals and virtually no background signal. We compare this technique to an existing approach, which has been widely adopted. We demonstrate how application of DMARCM in the gastrointestinal epithelium permits the effects of labeling frequency on tumorigenic stem cell growth to be distinguished in an established tumor model.

Original language	English
Pages (from-to)	9-12
Number of pages	4
Journal	Developmental Biology
Volume	457
Issue number	1
DOIs	https://doi.org/10.1016/j.ydbio.2019.09.008
State	Published - Jan 1 2020

Keywords

Cell lineage analysis
Drosophila
Monoclonality
Stem cells
Tumorigenesis

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10.1016/j.ydbio.2019.09.008

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KW - Monoclonality

KW - Stem cells

KW - Tumorigenesis

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Ultra-low frequency labeling of single cell lineages in Drosophila

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Keywords

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