Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts

Jesse D. Woodson, Matthew S. Joens, Andrew B. Sinson, Jonathan Gilkerson, Patrice A. Salomé, Detlef Weigel, James A. Fitzpatrick, Joanne Chory

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

Energy production by chloroplasts and mitochondria causes constant oxidative damage. A functioning photosynthetic cell requires quality-control mechanisms to turn over and degrade chloroplasts damaged by reactive oxygen species (ROS). Here, we generated a conditionally lethal Arabidopsis mutant that accumulated excess protoporphyrin IX in the chloroplast and produced singlet oxygen. Damaged chloroplasts were subsequently ubiquitinated and selectively degraded. A genetic screen identified the plant U-box 4 (PUB4) E3 ubiquitin ligase as being necessary for this process. pub4-6 mutants had defects in stress adaptation and longevity. Thus, we have identified a signal that leads to the targeted removal of ROS-overproducing chloroplasts.

Original languageEnglish
Pages (from-to)450-454
Number of pages5
JournalScience
Volume350
Issue number6259
DOIs
StatePublished - Oct 23 2015

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    Woodson, J. D., Joens, M. S., Sinson, A. B., Gilkerson, J., Salomé, P. A., Weigel, D., Fitzpatrick, J. A., & Chory, J. (2015). Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts. Science, 350(6259), 450-454. https://doi.org/10.1126/science.aac7444