TY - JOUR
T1 - U1 small nuclear RNA-like sequences in human high molecular weight RNA
AU - Capasso, J. M.
AU - Docherty, M. A.
AU - Ray, A.
AU - Kaplan, E. D.
AU - Eliceiri, G. L.
N1 - Funding Information:
ACKNO~LLDGbIENTS: ~e thank brs. T. l lanser and R.F. Gesteland for plasmid pD1, hrs. J.L. bahlberg and E. Lund for the human UI gene ~113 clone, and Drs. B.~I. O'halley and FI.J. Tsai for plasmid pU1 33. This work was supported by NIH grants CA27288, GM22463, CA20683 and RR05388.
PY - 1984/7/31
Y1 - 1984/7/31
N2 - Human U1 small nuclear RNA synthesis was shown earlier to be very sensitive to UV radiation. This led us to test for the possible presence of U1 RNA-like sequences in large RNAs. Human RNA was analyzed in gel blots hybridized with U1 DNA probes. A high molecular weight, heterodisperse RNA population was detected, which hybridizes both to a U1 RNA-coding region single-stranded DNA probe, and to a U1 gene fragment that contains only 6 nucleotides of flanking sequence. These large RNAs can be hybrid selected using immobilized U1 DNA, and have an average size of several kilobases. Additional observations support the claims that the high molecular weight RNA hybridization signal is not an aggregation artifact and that it is sequence specific.
AB - Human U1 small nuclear RNA synthesis was shown earlier to be very sensitive to UV radiation. This led us to test for the possible presence of U1 RNA-like sequences in large RNAs. Human RNA was analyzed in gel blots hybridized with U1 DNA probes. A high molecular weight, heterodisperse RNA population was detected, which hybridizes both to a U1 RNA-coding region single-stranded DNA probe, and to a U1 gene fragment that contains only 6 nucleotides of flanking sequence. These large RNAs can be hybrid selected using immobilized U1 DNA, and have an average size of several kilobases. Additional observations support the claims that the high molecular weight RNA hybridization signal is not an aggregation artifact and that it is sequence specific.
UR - http://www.scopus.com/inward/record.url?scp=0021774494&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(84)80110-2
DO - 10.1016/S0006-291X(84)80110-2
M3 - Article
C2 - 6205655
AN - SCOPUS:0021774494
VL - 122
SP - 838
EP - 844
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -