Tyrosine phosphorylation modifies protein kinase C δ-dependent phosphorylation of cardiac troponin I

Marius P. Sumandea, Vitalyi O. Rybin, Aaron C. Hinken, Chaojian Wang, Tomoyoshi Kobayashi, Erin Harleton, Gail Sievert, C. William Balke, Steven J. Feinmark, R. John Solaro, Susan F. Steinberg

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54 Scopus citations


Our study identifies tyrosine phosphorylation as a novel protein kinase Cδ (PKCδ) activation mechanism that modifies PKCδ-dependent phosphorylation of cardiac troponin I (cTnI), a myofilament regulatory protein. PKCδ phosphorylates cTnI at Ser23/Ser24 when activated by lipid cofactors; Src phosphorylates PKCδ at Tyr311 and Tyr332 leading to enhanced PKCδ autophosphorylation at Thr505 (its activation loop) and PKCδ-dependent cTnI phosphorylation at both Ser23/Ser24 and Thr144. The Src-dependent acquisition of cTnI-Thr144 kinase activity is abrogated by Y311F or T505A substitutions. Treatment of detergent-extracted single cardiomyocytes with lipid-activated PKCδ induces depressed tension at submaximum but not maximum [Ca2+] as expected for cTnI-Ser 23/Ser24 phosphorylation. Treatment of myocytes with Src-activated PKCδ leads to depressed maximum tension and cross-bridge kinetics, attributable to a dominant effect of cTnI-Thr144 phosphorylation. Our data implicate PKCδ-Tyr311/Thr 505 phosphorylation as dynamically regulated modifications that alter PKCδ enzymology and allow for stimulus-specific control of cardiac mechanics during growth factor stimulation and oxidative stress.

Original languageEnglish
Pages (from-to)22680-22689
Number of pages10
JournalJournal of Biological Chemistry
Issue number33
StatePublished - Aug 15 2008


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