Tyrosine phosphatase CD45 is required for T-cell antigen receptor and CD2-mediated activation of a protein tyrosine kinase and interleukin 2 production

G. A. Koretzky, J. Picus, T. Schultz, A. Weiss

Research output: Contribution to journalArticlepeer-review

323 Scopus citations

Abstract

CD45, a hematopoietic cell-specific surface antigen, has recently been shown to be a protein tyrosine phosphatase. Expression of CD45 is essential for the T-cell antigen receptor to couple with the phosphatidylinositol second messenger pathway and for antigen-mediated proliferation of T lymphocytes. In this report we describe a CD45- deficient mutant of the human T-cell leukemia line Jurkat. CD45 expression is required for the activation of a T-cell receptor- associated tyrosine kinase as well as the phosphatidylinositol pathway. Additionally, stimulation of T lymphocytes by way of the accessory molecule CD2 requires the expression of CD45. The mutation in the CD45-deficient cell specifically impairs signal transduction by the T-cell receptor and CD2 because activation events by way of another accessory molecule, CD28, are unimpaired.

Original languageEnglish
Pages (from-to)2037-2041
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number6
DOIs
StatePublished - 1991

Keywords

  • CD28
  • phosphatidylinositol
  • second messengers
  • signal transduction

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