Tyrosine kinase activation is necessary for inducible nitric oxide synthase expression by interleukin-1β

T. Tetsuka, A. R. Morrison

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The inflammatory cytokine interleukin-1 (IL-1) induces the inducible form of nitric oxide synthase (iNOS) with an increase in nitric oxide in rat mesangial cells. However, the cellular mechanisms that underlie the induction of iNOS by IL-1β in mesangial cells has not been clarified. Because we have shown that tyrosine kinase inhibitors attenuate IL-1β-induced cyclooxygenase expression and prostaglandin production, we investigated the effect of tyrosine kinase inhibitors on IL-1β-induced nitrite production and iNOS mRNA expression in rat mesangial cells. The tyrosine kinase inhibitors genistein and herbimycin A attenuated IL-1β-induced nitrite production in a dose- dependent manner. In addition, both of these inhibitors blocked IL-1β- induced iNOS mRNA expression. These data suggest that tyrosine kinase(s) plays a central role in IL-1β signaling to induce iNOS in rat mesangial cells.

Original languageEnglish
Pages (from-to)C55-C59
JournalAmerican Journal of Physiology - Cell Physiology
Volume269
Issue number1 38-1
StatePublished - Jan 1 1995

Keywords

  • interleukin-1
  • protein tyrosine kinase
  • signal transduction

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