Typing single-nucleotide polymorphisms in Toxoplasma gondii by allele-specific primer extension and microarray detection.

Chunlei Su, Christian Hott, Bernard H. Brownstein, L. David Sibley

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Genotyping is an important tool for epidemiological and population genetic studies in protozoan parasites. The most commonly used method for genotyping is polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) analysis of single nucleotide polymorphisms (SNPs). However, PCR-RFLP analysis is labor intensive, and only a proportion of the SNPs are recognized by currently available restriction enzymes. Here, we have developed a more efficient microarray-based method to genotype SNPs in the protozoan parasite Toxoplasma gondii. This method is sensitive, accurate, and capable of analyzing multiple SNPs simultaneously in a high-throughput format.

Original languageEnglish
Pages (from-to)249-262
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
Volume270
StatePublished - 2004

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