Two prostate carcinoma cell lines demonstrate abnormalities in tumor suppressor genes

Steven J. Rubin, Dennis E. Hallahan, Charles R. Ashman, David G. Brachman, Michael A. Beckett, Subbulakshmi Virudachalam, David W. Yandell, Ralph R. Weichselbaum

Research output: Contribution to journalArticlepeer-review

98 Scopus citations


Two prostate carcinoma cell lines, DU‐145 and PC‐3, were examined for abnormalities in the retinoblastoma (Rb) and the p53 putative tumor suppressor genes. We found an abnormal Rb gene product in DU‐145 using Western blot analysis. Polymerase chain reaction amplification followed by direct DNA sequencing demonstrated a base substitution mutation that generates a stop codon in exon 21. On Northern, Southern, and Western blot analysis, the p53 gene and its product appear to be normal in DU‐145. PC‐3, however, failed to demonstrate expression of either the p53 transcript on Northern blot analysis or the p53 protein on Western blot analysis, while the Rb gene products appeared to be normal on both Northern and Western blot analysis. This work extends the correlation between abnormal expression of putative tumor suppressor genes and human malignancies.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalJournal of surgical oncology
Issue number1
StatePublished - Jan 1991


  • Western blot
  • p53
  • polymerase chain reaction
  • retinoblastoma


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