TY - JOUR
T1 - Two-photon phosphorescence lifetime microscopy of retinal capillary plexus oxygenation in mice
AU - Şencan, Ikbal
AU - Esipova, Tatiana V.
AU - Yaseen, Mohammad A.
AU - Fu, Buyin
AU - Boas, David A.
AU - Vinogradov, Sergei A.
AU - Shahidi, Mahnaz
AU - Sakadžić, Sava
N1 - Publisher Copyright:
© The Authors.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Impaired oxygen delivery and/or consumption in the retinal tissue underlies the pathophysiology of many retinal diseases. However, the essential tools for measuring oxygen concentration in retinal capillaries and studying oxygen transport to retinal tissue are still lacking. We show that two-photon phosphorescence lifetime microscopy can be used to map absolute partial pressures of oxygen (pO 2) in the retinal capillary plexus. Measurements were performed at various retinal depths in anesthetized mice under systemic normoxic and hyperoxic conditions. We used a newly developed two-photon phosphorescent oxygen probe, based on a two-photon absorbing platinum tetraphthalimidoporphyrin, and commercially available optics without correction for optical aberrations of the eye. The transverse and axial distances within the tissue volume were calibrated using a model of the eye's optical system. We believe this is the first demonstration of in vivo depth-resolved imaging of pO 2 in retinal capillaries. Application of this method has the potential to advance our understanding of oxygen delivery on the microvascular scale and help elucidate mechanisms underlying various retinal diseases.
AB - Impaired oxygen delivery and/or consumption in the retinal tissue underlies the pathophysiology of many retinal diseases. However, the essential tools for measuring oxygen concentration in retinal capillaries and studying oxygen transport to retinal tissue are still lacking. We show that two-photon phosphorescence lifetime microscopy can be used to map absolute partial pressures of oxygen (pO 2) in the retinal capillary plexus. Measurements were performed at various retinal depths in anesthetized mice under systemic normoxic and hyperoxic conditions. We used a newly developed two-photon phosphorescent oxygen probe, based on a two-photon absorbing platinum tetraphthalimidoporphyrin, and commercially available optics without correction for optical aberrations of the eye. The transverse and axial distances within the tissue volume were calibrated using a model of the eye's optical system. We believe this is the first demonstration of in vivo depth-resolved imaging of pO 2 in retinal capillaries. Application of this method has the potential to advance our understanding of oxygen delivery on the microvascular scale and help elucidate mechanisms underlying various retinal diseases.
KW - oxygen partial pressure
KW - phosphorescence quenching
KW - retinal imaging
KW - two-photon microscopy
UR - http://www.scopus.com/inward/record.url?scp=85058315119&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.23.12.126501
DO - 10.1117/1.JBO.23.12.126501
M3 - Article
C2 - 30516039
AN - SCOPUS:85058315119
SN - 1083-3668
VL - 23
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 12
M1 - 126501
ER -