Two-photon optical properties of near-infrared dyes at 1.55 μm excitation

Mikhail Y. Berezin; Chun Zhan; Hyeran Lee; Chulmin Joo; Walter J. Akers; Siavash Yazdanfar; Samuel Achilefu

doi:10.1021/jp207618e

Two-photon optical properties of near-infrared dyes at 1.55 μm excitation

Mikhail Y. Berezin, Chun Zhan, Hyeran Lee, Chulmin Joo, Walter J. Akers, Siavash Yazdanfar, Samuel Achilefu

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Abstract

Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC, showed the highest 2P action cross-section (∼103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors. Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.

Original language	English
Pages (from-to)	11530-11535
Number of pages	6
Journal	Journal of Physical Chemistry B
Volume	115
Issue number	39
DOIs	https://doi.org/10.1021/jp207618e
State	Published - Oct 6 2011

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title = "Two-photon optical properties of near-infrared dyes at 1.55 μm excitation",

abstract = "Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC, showed the highest 2P action cross-section (∼103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors. Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.",

author = "Berezin, {Mikhail Y.} and Chun Zhan and Hyeran Lee and Chulmin Joo and Akers, {Walter J.} and Siavash Yazdanfar and Samuel Achilefu",

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doi = "10.1021/jp207618e",

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T1 - Two-photon optical properties of near-infrared dyes at 1.55 μm excitation

AU - Berezin, Mikhail Y.

AU - Zhan, Chun

AU - Lee, Hyeran

AU - Joo, Chulmin

AU - Akers, Walter J.

AU - Yazdanfar, Siavash

AU - Achilefu, Samuel

PY - 2011/10/6

Y1 - 2011/10/6

N2 - Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC, showed the highest 2P action cross-section (∼103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors. Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.

AB - Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC, showed the highest 2P action cross-section (∼103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors. Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.

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ER -

Two-photon optical properties of near-infrared dyes at 1.55 μm excitation

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