TY - JOUR
T1 - Two-photon microscopy of host-pathogen interactions
T2 - Acquiring a dynamic picture of infection in vivo
AU - Konjufca, Vjollca
AU - Miller, Mark J.
PY - 2009
Y1 - 2009
N2 - Two-photon (2P) microscopy has become increasingly popular among immunologists for analysing single-cell dynamics in tissues. Researchers are now taking 2P microscopy beyond the study of model antigen systems (e.g. ovalbumin immunization) and are applying the technique to examine infection in vivo. With the appropriate fluorescent probes, 2P imaging can provide high-resolution spatio-temporal information regarding cell behaviour, monitor cell functions and assess various outcomes of infection, such as host cell apoptosis or pathogen proliferation. Imaging of transgenic and knockout mice can be used to probe molecular mechanisms governing the host response to infection. From the microbe side, imaging genetically engineered mutant strains of a pathogen can test the roles of specific virulence factors in pathogenesis. Here, we discuss recent work that has applied 2P microscopy to study models of infection and highlight the tremendous potential that this approach has for investigating host-pathogen interactions.
AB - Two-photon (2P) microscopy has become increasingly popular among immunologists for analysing single-cell dynamics in tissues. Researchers are now taking 2P microscopy beyond the study of model antigen systems (e.g. ovalbumin immunization) and are applying the technique to examine infection in vivo. With the appropriate fluorescent probes, 2P imaging can provide high-resolution spatio-temporal information regarding cell behaviour, monitor cell functions and assess various outcomes of infection, such as host cell apoptosis or pathogen proliferation. Imaging of transgenic and knockout mice can be used to probe molecular mechanisms governing the host response to infection. From the microbe side, imaging genetically engineered mutant strains of a pathogen can test the roles of specific virulence factors in pathogenesis. Here, we discuss recent work that has applied 2P microscopy to study models of infection and highlight the tremendous potential that this approach has for investigating host-pathogen interactions.
UR - http://www.scopus.com/inward/record.url?scp=62149109716&partnerID=8YFLogxK
U2 - 10.1111/j.1462-5822.2009.01289.x
DO - 10.1111/j.1462-5822.2009.01289.x
M3 - Review article
C2 - 19170686
AN - SCOPUS:62149109716
SN - 1462-5814
VL - 11
SP - 551
EP - 559
JO - Cellular microbiology
JF - Cellular microbiology
IS - 4
ER -