TY - JOUR
T1 - Two-photon microperimetry
T2 - Sensitivity of human photoreceptors to infrared light
AU - Ruminski, Daniel
AU - Palczewska, Grazyna
AU - Nowakowski, Maciej
AU - Zielińska, Agnieszka
AU - Kefalov, Vladimir J.
AU - Komar, Katarzyna
AU - Palczewski, Krzysztof
AU - Wojtkowski, Maciej
N1 - Funding Information:
M.W. acknowledges to: European Union, Polish Ministry of Science and Foundation for Polish Science. K.P. acknowledges to National Institutes of Health. K.P. is Chief Scientific Officer of Polgenix, Inc. K.K. acknowledges to the National Science Centre and to the City of Gdynia. V.J.K. acknowledges to the Department of Ophthalmology and Visual Sciences at Washington University and by Research to Prevent Blindness. We thank the members of the Palczewski laboratory for valuable comments regarding this manuscript. This work was partially carried out at the Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio, USA. We also acknowledge Krzysztof Dalasinski for technical support, Karolina Kiluk for help with the dark adaptation measurements, and the contribution of human donor eye lenses from Dr. Irina Pikuleva.
Funding Information:
National Institutes of Health (EY019312, EY025451, EY027283); Research to Prevent Blindness; Gavin Herbert Eye Institute and the Department of Ophthalmology, University of California, Irvine; Department of Ophthalmology and Visual Sciences at Washington University; Narodowe Centrum Nauki (2016/23/B/ST2/00752); City of Gdynia (3/DOT/2016); Horizon 2020 Framework Programme (666295); Fundacja na rzecz Nauki Polskiej (TEAM TECH/2016-3/20). M.W. acknowledges to: European Union, Polish Ministry of Science and Foundation for Polish Science. K.P. acknowledges to National Institutes of Health. K.P. is Chief Scientific Officer of Polgenix, Inc. K.K. acknowledges to the National Science Centre and to the City of Gdynia. V.J.K. acknowledges to the Department of Ophthalmology and Visual Sciences at Washington University and by Research to Prevent Blindness. We thank the members of the Palczewski laboratory for valuable comments regarding this manuscript. This work was partially carried out at the Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio, USA. We also acknowledge Krzysztof Dalasinski for technical support, Karolina Kiluk for help with the dark adaptation measurements, and the contribution of human donor eye lenses from Dr. Irina Pikuleva.
Publisher Copyright:
© 2019 Optical Society of America.
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Microperimetry is a subjective ophthalmologic test used to assess retinal function at various specific and focal locations of the visual field. Historically, visible light has been described as ranging from 400 to 720 nm. However, we previously demonstrated that infra-red light can initiate visual transduction in rod photoreceptors by a mechanism of two-photon absorption by visual pigments. Here we introduce a newly designed and constructed two-photon microperimeter. We provide for the first time evidence of the presence of a nonlinear process occurring in the human retina based on psychophysical tests using newly developed instrumentation. Since infra-red light penetrates the aged front of the eye better than visible light, it has the potential for improved functional diagnostics in patients with age-related visual disorders.
AB - Microperimetry is a subjective ophthalmologic test used to assess retinal function at various specific and focal locations of the visual field. Historically, visible light has been described as ranging from 400 to 720 nm. However, we previously demonstrated that infra-red light can initiate visual transduction in rod photoreceptors by a mechanism of two-photon absorption by visual pigments. Here we introduce a newly designed and constructed two-photon microperimeter. We provide for the first time evidence of the presence of a nonlinear process occurring in the human retina based on psychophysical tests using newly developed instrumentation. Since infra-red light penetrates the aged front of the eye better than visible light, it has the potential for improved functional diagnostics in patients with age-related visual disorders.
UR - http://www.scopus.com/inward/record.url?scp=85078707687&partnerID=8YFLogxK
U2 - 10.1364/BOE.10.004551
DO - 10.1364/BOE.10.004551
M3 - Article
C2 - 31565509
AN - SCOPUS:85078707687
SN - 2156-7085
VL - 10
SP - 4551
EP - 4567
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 9
ER -