Two-photon imaging of lymphocyte motility and antigen response in intact lymph node

Mark J. Miller, Sindy H. Wei, Ian Parker, Michael D. Cahalan

Research output: Contribution to journalArticle

913 Scopus citations

Abstract

Lymphocyte motility is vital for trafficking within lymphoid organs and for initiating contact with antigen-presenting cells. Visualization of these processes has previously been limited to in vitro systems. We describe the use of two-photon laser microscopy to image the dynamic behavior of individual living lymphocytes deep within intact lymph nodes. In their native environment, T cells achieved peak velocities of more than 25 micrometers per minute, displaying a motility coefficient that is five to six times that of B cells. Antigenic challenge changed T cell trajectories from random walks to "swarms" and stable clusters. Real-time two-photon imaging reveals lymphocyte behaviors that are fundamental to the initiation of the immune response.

Original languageEnglish
Pages (from-to)1869-1873
Number of pages5
JournalScience
Volume296
Issue number5574
DOIs
StatePublished - Jun 7 2002

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