@article{eb2285e6a22b42b2ae34994ca3e5b240,
title = "Tweek, an Evolutionarily Conserved Protein, Is Required for Synaptic Vesicle Recycling",
abstract = "Synaptic vesicle endocytosis is critical for maintaining synaptic communication during intense stimulation. Here we describe Tweek, a conserved protein that is required for synaptic vesicle recycling. tweek mutants show reduced FM1-43 uptake, cannot maintain release during intense stimulation, and harbor larger than normal synaptic vesicles, implicating it in vesicle recycling at the synapse. Interestingly, the levels of a fluorescent PI(4,5)P2 reporter are reduced at tweek mutant synapses, and the probe is aberrantly localized during stimulation. In addition, various endocytic adaptors known to bind PI(4,5)P2 are mislocalized and the defects in FM1-43 dye uptake and adaptor localization are partially suppressed by removing one copy of the phosphoinositide phosphatase synaptojanin, suggesting a role for Tweek in maintaining proper phosphoinositide levels at synapses. Our data implicate Tweek in regulating synaptic vesicle recycling via an action mediated at least in part by the regulation of PI(4,5)P2 levels or availability at the synapse.",
keywords = "CELLBIO, MOLNEURO, SIGNALING",
author = "Patrik Verstreken and Tomoko Ohyama and Claire Haueter and Habets, \{Ron L.P.\} and Lin, \{Yong Q.\} and Swan, \{Laura E.\} and Ly, \{Cindy V.\} and Venken, \{Koen J.T.\} and \{De Camilli\}, Pietro and Bellen, \{Hugo J.\}",
note = "Funding Information: We are grateful to the Bloomington Stock Center, the Developmental Studies Hybridoma Bank, VDRC, Kwang Choi, Aaron Di Antonio, Barry Dickson, Bruce Edgar, Marcos Gonz{\'a}lez-Gait{\'a}n, Leonard Kelly, Jeanette Kunz, Cahir O'Kane, and Bing Zhang for reagents. We thank Yuchun He for injections, Shinya Yamamoto, Karen Schulze, Elaine Seto, Nikolaos Giagtzoglou, Hiroshi Tsuda, Gilbert Di Paolo, Jeanette Kunz, and members of the Bellen, Verstreken, and De Camilli labs for comments. Confocal microscopy was supported by the Baylor College of Medicine Intellectual and Developmental Disabilities Research Center and the K.U.Leuven Light Microscopy Network (LiMoNe). P.V. was supported by an R.L. Kirchstein NRS award, a Marie Curie Excellence Grant (MEXT-CT-2006-042267), the Research Fund K.U.Leuven, FWO grant G.0747.09, and VIB; C.V.L. was supported by an NRS award; and P.D.C. and H.J.B. are HHMI investigators. ",
year = "2009",
month = jul,
day = "30",
doi = "10.1016/j.neuron.2009.06.017",
language = "English",
volume = "63",
pages = "203--215",
journal = "Neuron",
issn = "0896-6273",
number = "2",
}