tsBN75 and tsBN423, temperature-sensitive X-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA

Hideo Nishitani; Hiroshige Goto; Sumiko Kaneda; Fumiaki Yamao; Takesi Seno; Patricia Handley; Alan L. Schwartz; Takeharu Nishimoto

doi:10.1016/0006-291X(92)90692-E

tsBN75 and tsBN423, temperature-sensitive X-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA

Hideo Nishitani, Hiroshige Goto, Sumiko Kaneda, Fumiaki Yamao, Takesi Seno, Patricia Handley, Alan L. Schwartz, Takeharu Nishimoto

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

tsBN75 and tsBN423 are independently isolated temperature-sensitive (ts) mutants of the BHK21 cell line for cell growth. Both tsBN75 and tsBN423 belong to the same complementation group and show G2 block at the nonpermissive temperature. Both were efficiently transformed to ts⁺ cells with the mouse and human cDNA encoding the ubiquitin-activating enzyme, E1. While no transformants of tsBN423 cells had a DNA content greater than the parental 2C, several ts⁺ transformants of tsBN75 cells acquired a multiploid DNA content. These data thus demonstrate the function of the human and mouse E1 cDNAs and further suggest that E1 functions in more than one step in cell cycle progression.

Original language	English
Pages (from-to)	1015-1021
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	184
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(92)90692-E
State	Published - Apr 30 1992

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Nishitani, H., Goto, H., Kaneda, S., Yamao, F., Seno, T., Handley, P., Schwartz, A. L., & Nishimoto, T. (1992). tsBN75 and tsBN423, temperature-sensitive X-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA. Biochemical and Biophysical Research Communications, 184(2), 1015-1021. https://doi.org/10.1016/0006-291X(92)90692-E

@article{4c5022ce944343478670361247c26d02,

title = "tsBN75 and tsBN423, temperature-sensitive X-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA",

abstract = "tsBN75 and tsBN423 are independently isolated temperature-sensitive (ts) mutants of the BHK21 cell line for cell growth. Both tsBN75 and tsBN423 belong to the same complementation group and show G2 block at the nonpermissive temperature. Both were efficiently transformed to ts+ cells with the mouse and human cDNA encoding the ubiquitin-activating enzyme, E1. While no transformants of tsBN423 cells had a DNA content greater than the parental 2C, several ts+ transformants of tsBN75 cells acquired a multiploid DNA content. These data thus demonstrate the function of the human and mouse E1 cDNAs and further suggest that E1 functions in more than one step in cell cycle progression.",

author = "Hideo Nishitani and Hiroshige Goto and Sumiko Kaneda and Fumiaki Yamao and Takesi Seno and Patricia Handley and Schwartz, {Alan L.} and Takeharu Nishimoto",

note = "Funding Information: This work was supported by Grants-in Aid for Scientific Research and for Cancer Research from the Ministry of Education, Science and Culture and from the Science and Technology Agency, and by the Mitsubishi foundation.",

year = "1992",

month = apr,

day = "30",

doi = "10.1016/0006-291X(92)90692-E",

language = "English",

volume = "184",

pages = "1015--1021",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

number = "2",

}

Nishitani, H, Goto, H, Kaneda, S, Yamao, F, Seno, T, Handley, P, Schwartz, AL & Nishimoto, T 1992, 'tsBN75 and tsBN423, temperature-sensitive X-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA', Biochemical and Biophysical Research Communications, vol. 184, no. 2, pp. 1015-1021. https://doi.org/10.1016/0006-291X(92)90692-E

tsBN75 and tsBN423, temperature-sensitive X-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA. / Nishitani, Hideo; Goto, Hiroshige; Kaneda, Sumiko et al.
In: Biochemical and Biophysical Research Communications, Vol. 184, No. 2, 30.04.1992, p. 1015-1021.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - tsBN75 and tsBN423, temperature-sensitive X-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA

AU - Nishitani, Hideo

AU - Goto, Hiroshige

AU - Kaneda, Sumiko

AU - Yamao, Fumiaki

AU - Seno, Takesi

AU - Handley, Patricia

AU - Schwartz, Alan L.

AU - Nishimoto, Takeharu

N1 - Funding Information: This work was supported by Grants-in Aid for Scientific Research and for Cancer Research from the Ministry of Education, Science and Culture and from the Science and Technology Agency, and by the Mitsubishi foundation.

PY - 1992/4/30

Y1 - 1992/4/30

N2 - tsBN75 and tsBN423 are independently isolated temperature-sensitive (ts) mutants of the BHK21 cell line for cell growth. Both tsBN75 and tsBN423 belong to the same complementation group and show G2 block at the nonpermissive temperature. Both were efficiently transformed to ts+ cells with the mouse and human cDNA encoding the ubiquitin-activating enzyme, E1. While no transformants of tsBN423 cells had a DNA content greater than the parental 2C, several ts+ transformants of tsBN75 cells acquired a multiploid DNA content. These data thus demonstrate the function of the human and mouse E1 cDNAs and further suggest that E1 functions in more than one step in cell cycle progression.

AB - tsBN75 and tsBN423 are independently isolated temperature-sensitive (ts) mutants of the BHK21 cell line for cell growth. Both tsBN75 and tsBN423 belong to the same complementation group and show G2 block at the nonpermissive temperature. Both were efficiently transformed to ts+ cells with the mouse and human cDNA encoding the ubiquitin-activating enzyme, E1. While no transformants of tsBN423 cells had a DNA content greater than the parental 2C, several ts+ transformants of tsBN75 cells acquired a multiploid DNA content. These data thus demonstrate the function of the human and mouse E1 cDNAs and further suggest that E1 functions in more than one step in cell cycle progression.

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DO - 10.1016/0006-291X(92)90692-E

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SN - 0006-291X

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

tsBN75 and tsBN423, temperature-sensitive X-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA

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