TY - JOUR
T1 - TRPV4 acts as a mitochondrial Ca2+-importer and regulates mitochondrial temperature and metabolism
AU - Kanta Acharya, Tusar
AU - Kumar, Ashutosh
AU - Kumar Majhi, Rakesh
AU - Kumar, Shamit
AU - Chakraborty, Ranabir
AU - Tiwari, Ankit
AU - Smalla, Karl Heinz
AU - Liu, Xiao
AU - Chang, Young Tae
AU - Gundelfinger, Eckart D.
AU - Goswami, Chandan
N1 - Funding Information:
Funding from NISER and DBT (Govt. India, grant number BT-BRB-TF-2-2011 & BT/PR8004/MED/30/988/2013) are acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Imaging facility and animal house facility of NISER is appreciated. CG acknowledge the previous supports from Max Planck Institute for Molecular Genetics, Berlin and Dr. A. Santle for help with HSP-60 antibody. CG acknowledges all the present and former lab members for supporting this work. The MTY, ETY, ATP Red dye and GTP Green dye were synthesized with the help of IBS-R007-A1 given to YTC.
Funding Information:
Funding from NISER and DBT (Govt. India, grant number BT-BRB-TF-2-2011 & BT/PR8004/MED/30/988/2013) are acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Imaging facility and animal house facility of NISER is appreciated. CG acknowledge the previous supports from Max Planck Institute for Molecular Genetics, Berlin and Dr. A. Santle for help with HSP-60 antibody. CG acknowledges all the present and former lab members for supporting this work. The MTY, ETY, ATP Red dye and GTP Green dye were synthesized with the help of IBS-R007-A1 given to YTC.
Publisher Copyright:
© 2022 Elsevier B.V. and Mitochondria Research Society
PY - 2022/11
Y1 - 2022/11
N2 - TRPV4 is associated with the development of neuropathic pain, sensory defects, muscular dystrophies, neurodegenerative disorders, Charcot Marie Tooth and skeletal dysplasia. In all these cases, mitochondrial abnormalities are prominent. Here, we demonstrate that TRPV4, localizes to a subpopulation of mitochondria in various cell lines. Improper expression and/or function of TRPV4 induces several mitochondrial abnormalities. TRPV4 is also involved in the regulation of mitochondrial numbers, Ca2+-levels and mitochondrial temperature. Accordingly, several naturally occurring TRPV4 mutations affect mitochondrial morphology and distribution. These findings may help in understanding the significance of mitochondria in TRPV4-mediated channelopathies possibly classifying them as mitochondrial diseases.
AB - TRPV4 is associated with the development of neuropathic pain, sensory defects, muscular dystrophies, neurodegenerative disorders, Charcot Marie Tooth and skeletal dysplasia. In all these cases, mitochondrial abnormalities are prominent. Here, we demonstrate that TRPV4, localizes to a subpopulation of mitochondria in various cell lines. Improper expression and/or function of TRPV4 induces several mitochondrial abnormalities. TRPV4 is also involved in the regulation of mitochondrial numbers, Ca2+-levels and mitochondrial temperature. Accordingly, several naturally occurring TRPV4 mutations affect mitochondrial morphology and distribution. These findings may help in understanding the significance of mitochondria in TRPV4-mediated channelopathies possibly classifying them as mitochondrial diseases.
KW - Mitochondrial Ca
KW - Mitochondrial dynamics
KW - Mitochondrial temperature
KW - Neuro-Muscular Dystrophies Mitochondrial diseases
KW - TRP channels
UR - http://www.scopus.com/inward/record.url?scp=85140319599&partnerID=8YFLogxK
U2 - 10.1016/j.mito.2022.10.001
DO - 10.1016/j.mito.2022.10.001
M3 - Article
C2 - 36261119
AN - SCOPUS:85140319599
SN - 1567-7249
VL - 67
SP - 38
EP - 58
JO - Mitochondrion
JF - Mitochondrion
ER -