TY - JOUR
T1 - Tropoelastin and fibulin overexpression in the subepithelial connective tissue of human pterygium
AU - Prez-Rico, Consuelo
AU - Pascual, Gemma
AU - Sotomayor, Sandra
AU - Montes-Molln, Mara Ngeles
AU - Trejo, Cynthia
AU - Sasaki, Takako
AU - Mecham, Robert
AU - Belln, Juan Manuel
AU - Bujn, Julia
N1 - Funding Information:
Publication of this article was supported by Grant RD07/0062/0008 from the FundaciÓn para la Investigación Biomédica del Hospital Universitario Príncipe de Asturias (Alcalá de Henares, Spain), and Instituto de Salud Carlos III (Madrid, Spain). The authors indicate no financial conflict of interest. Involved in Design and conduct of study (C.P-R., G.P., J.B.); Collection, management, analysis, and interpretation of data (G.P., S.S., M.A.M-M., C.T., C.P-R.); Preparation of article (C.P-R., G.P.); and Review and approval of the manuscript (J.M.B., T.S., R.M., J.B.). The study protocol was approved by the Ethics Committee of the University Hospital Principe de Asturias, and all study procedures adhered to the recommendations of the Declaration of Helsinki. Written consent was obtained from all participants.
PY - 2011/1
Y1 - 2011/1
N2 - Purpose To evaluate possible changes in the collagen and elastic components of the subepithelial connective tissue of human pterygium. Design Immunohistochemical study. Methods Immunohistochemical staining using antitropoelastin, anti-fibulin-2, and anti-fibulin-3 antibodies was performed in 10 normal conjunctival and 20 pterygium specimens. Masson trichome staining also was performed to study subepithelial connective tissue. Sirius red staining was used to identify collagen type I and III components. Tropoelastin, fibulin-2, and fibulin-3 messenger ribonucleic acid (mRNA) expressions were analyzed in 9 conjunctival and 12 pterygium specimens by quantitative real-time polymerase chain reaction assay. Results The subepithelial connective tissue and vessels were more predominant in pterygium compared with the normal conjunctival tissue. Amorphous subepithelial zones were observed in the areas of the pterygium tissue, but not in normal conjunctiva. Increased tropoelastin staining was seen in the pterygium tissue with areas of degenerative changes or immature formation of elastic fibers, as well an increase in tropoelastin mRNA, in contrast with fibulin-2 and fibulin-3 messenger levels. Fibulin-2 and fibulin-3 expression was colocalized in the subepithelial connective tissue and was distributed along blood and lymphatic vessels. Collagen type III, an immature form of collagen, was increased in the pathologic samples in association with a tissue remodeling process. Conclusions Elastin metabolism is dysregulated in the pathogenesis of human pterygium with tropoelastin, fibulin-2, and fibulin-3 overexpression in the subepithelial connective tissue.
AB - Purpose To evaluate possible changes in the collagen and elastic components of the subepithelial connective tissue of human pterygium. Design Immunohistochemical study. Methods Immunohistochemical staining using antitropoelastin, anti-fibulin-2, and anti-fibulin-3 antibodies was performed in 10 normal conjunctival and 20 pterygium specimens. Masson trichome staining also was performed to study subepithelial connective tissue. Sirius red staining was used to identify collagen type I and III components. Tropoelastin, fibulin-2, and fibulin-3 messenger ribonucleic acid (mRNA) expressions were analyzed in 9 conjunctival and 12 pterygium specimens by quantitative real-time polymerase chain reaction assay. Results The subepithelial connective tissue and vessels were more predominant in pterygium compared with the normal conjunctival tissue. Amorphous subepithelial zones were observed in the areas of the pterygium tissue, but not in normal conjunctiva. Increased tropoelastin staining was seen in the pterygium tissue with areas of degenerative changes or immature formation of elastic fibers, as well an increase in tropoelastin mRNA, in contrast with fibulin-2 and fibulin-3 messenger levels. Fibulin-2 and fibulin-3 expression was colocalized in the subepithelial connective tissue and was distributed along blood and lymphatic vessels. Collagen type III, an immature form of collagen, was increased in the pathologic samples in association with a tissue remodeling process. Conclusions Elastin metabolism is dysregulated in the pathogenesis of human pterygium with tropoelastin, fibulin-2, and fibulin-3 overexpression in the subepithelial connective tissue.
UR - http://www.scopus.com/inward/record.url?scp=78650509929&partnerID=8YFLogxK
U2 - 10.1016/j.ajo.2010.07.012
DO - 10.1016/j.ajo.2010.07.012
M3 - Article
C2 - 21094933
AN - SCOPUS:78650509929
SN - 0002-9394
VL - 151
SP - 44
EP - 52
JO - American journal of ophthalmology
JF - American journal of ophthalmology
IS - 1
ER -