Troglitazone attenuates hypoxia-induced injury in cultured term human trophoblasts

Uriel Elchalal, Rachel G. Humphrey, Steven D. Smith, Chaobin Hu, Yoel Sadovsky, D. Michael Nelson

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The purpose of this study was to test the hypothesis that the thiazolidinedione troglitazone, a peroxisome proliferator activated receptor-γ ligand, attenuates hypoxia-induced trophoblast injury. Cytotrophoblasts from 4 term human placentas were cultured in the presence or absence of 10 μmol/L troglitazone in either 20% oxygen (standard conditions) or 1% oxygen (hypoxic conditions) for variable periods before cell harvest. Medium β-human chorionic gonadotropin and human placental lactogen were analyzed by enzyme-linked immunosorbent assay. Apoptosis was quantified by cytokeratin-18 cleavage products staining; p53 expression was examined by Western blot analysis. β-human chorionic gonadotropin and human placental lactogen levels were ≥2-fold higher in troglitazone-exposed cells at 16 hours of hypoxia, compared with vehicle control cells (P <.05). The apoptotic index was reduced by ≥30% (P <.001), and the expression of p53 was 2-fold lower (P <.02) in troglitazone-exposed cells under hypoxia for ≤16 hours but not different after >24 hours of low oxygen. Troglitazone attenuates the influence of acute hypoxia on cultured term human trophoblasts.

Original languageEnglish
Pages (from-to)2154-2159
Number of pages6
JournalAmerican journal of obstetrics and gynecology
Volume191
Issue number6
DOIs
StatePublished - Dec 2004

Keywords

  • Apoptosis
  • Hypoxia
  • Peroxisome proliferator activated eceptor-γ
  • Placenta
  • Troglitazone

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