Triple Resonance Isotope-Edited (TRIED): A powerful new NMR technique for studying metabolism

W. C. Hutton, J. J. Likos, J. K. Gard, J. R. Garbow

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A powerful new technique for studying metabolism, Triple-Resonance Isotope-Edited (TRIED) NMR, is described and demonstrated experimentally. The experiment uses through-bond (scalar) coupling between spins to select NMR signals from molecules having 1H-13C-15N labeled triples. Natural-abundance background signal from molecules not containing such labeled triples (e.g., signal from 1H-12C and 1H-13C fragments) is effectively suppressed, with a suppression ratio of approximately 104:1. This effective filtering of signal from labeled molecules allows these species and their metabolites to be detected and characterized without requiring extensive, time-consuming sample purification. Results are presented for 13C,15N-aminomethylphosphonate (AMPA) in soybean and pigweed plant matrices. The potential use of TRIED for studying the pharmacokinetics of drugs and extension of the experiment to include the use of magnetic-field gradients for selecting signal from labeled fragments are also described.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume41
Issue number2
DOIs
StatePublished - Feb 1 1998
Externally publishedYes

Keywords

  • Carbon-13
  • Metabolism
  • Nitrogen-15
  • Nuclear magnetic resonance
  • Pharmacokinetics
  • Stable isotopes

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