Trimodal color-fluorescence-polarization endoscopy aided by a tumor selective molecular probe accurately detects flat lesions in colitis-associated cancer

Tauseef Charanya, Timothy York, Sharon Bloch, Gail Sudlow, Kexian Liang, Missael Garcia, Walter J. Akers, Deborah Rubin, Viktor Gruev, Samuel Achilefu

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Colitis-associated cancer (CAC) arises from premalignant flat lesions of the colon, which are difficult to detect with current endoscopic screening approaches. We have developed a complementary fluorescence and polarization reporting strategy that combines the unique biochemical and physical properties of dysplasia and cancer for real-time detection of these lesions. Using azoxymethane-dextran sodium sulfate (AOM-DSS) treated mice, which recapitulates human CAC and dysplasia, we show that an octapeptide labeled with a near-infrared (NIR) fluorescent dye selectively identified all precancerous and cancerous lesions. A new thermoresponsive sol-gel formulation allowed topical application of the molecular probe during endoscopy. This method yielded high contrast-to-noise ratios (CNR) between adenomatous tumors (20.6 ± 1.65) and flat lesions (12.1 ± 1.03) and surrounding uninvolved colon tissue versus CNR of inflamed tissues (1.62 ± 0.41). Incorporation of nanowire- filtered polarization imaging into NIR fluorescence endoscopy shows a high depolarization contrast in both adenomatous tumors and flat lesions in CAC, reflecting compromised structural integrity of these tissues. Together, the real-time polarization imaging provides real-time validation of suspicious colon tissue highlighted by molecular fluorescence endoscopy.

Original languageEnglish
Article number126002
JournalJournal of biomedical optics
Volume19
Issue number12
DOIs
StatePublished - Dec 1 2014

Keywords

  • colitis-associated cancer
  • endoscopy
  • flat lesions
  • fluorescence polarization
  • molecular imaging
  • near-infrared fluorescence
  • surveillance endoscopy
  • topical administration

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