TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47

Fabio L. Urbina, Shalini Menon, Dennis Goldfarb, Reginald Edwards, M. Ben Major, Patrick Brennwald, Stephanie L. Gupton

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Neuronal morphogenesis involves dramatic plasma membrane expansion, fueled by soluble N-ethylmaleimide-sensitive factor attachment protein eceptors (SNARE)-mediated exocytosis. Distinct fusion modes described at synapses include full-vesicle fusion (FVF) and kiss-and-run fusion (KNR). During FVF, lumenal cargo is secreted and vesicle membrane incorporates into the plasma membrane. During KNR, a transient fusion pore secretes cargo but closes without membrane addition. In contrast, fusion modes are not described in developing neurons. Here, we resolve individual exocytic events in developing murine cortical neurons and use classification tools to identify four distinguishable fusion modes: two FVF-like modes that insert membrane material and two KNR-like modes that do not. Discrete fluorescence profiles suggest distinct behavior of the fusion pore. Simulations and experiments agree that FVF-like exocytosis provides sufficient membrane material for morphogenesis. We find the E3 ubiquitin ligase TRIM67 promotes FVF-like exocytosis in part by limiting incorporation of the Qb/Qc SNARE SNAP47 into SNARE complexes and, thus, SNAP47 involvement in exocytosis.

Original languageEnglish
Article number108743
JournalCell Reports
Issue number6
StatePublished - Feb 9 2021


  • TIRF
  • classification
  • clustering
  • exocytosis
  • fusion
  • mode
  • morphogenesis
  • neuron
  • plasma membrane


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