TREM-like transcript 2 is stored in human neutrophil primary granules and is up-regulated in response to inflammatory mediators

Kimberly A. Thomas, R. Glenn King, Christine M. Sestero, Louis B. Justement

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The triggering receptor expressed on myeloid cell locus encodes a family of receptors that is emerging as an important class of molecules involved in modulating the innate immune response and inflammation. Of the 4 conserved members, including triggering receptor expressed on myeloid cells 1 and 2 and triggering receptor expressed on myeloid cell-like transcripts 1 and 2, relatively little is known about triggering receptor expressed on myeloid cell-like transcript 2 expression and function, particularly in humans. In this study, experiments were performed to determine if triggering receptor expressed on myeloid cell-like transcript 2 expression is conserved between mouse and human, demonstrating that human triggering receptor expressed on myeloid cell-like transcript 2 is expressed on cells of the lymphoid, as well as myeloid/granuloid lineages, similar to murine triggering receptor expressed on myeloid cell-like transcript 2. Consistent with studies in the mouse, triggering receptor expressed on myeloid cell-like transcript 2 expression is up-regulated in response to inflammatory mediators on human neutrophils. Importantly, it was shown that triggering receptor expressed on myeloid cell-like transcript 2, in resting human neutrophils, is predominantly localized to intracellular vesicles, including secretory vesicles and primary granules; with the majority of triggering receptor expressed on myeloid cell-like transcript 2 stored in primary granules. In contrast to other primary granule proteins, triggering receptor expressed on myeloid celllike transcript 2 is not expelled on neutrophil extracellular traps but is retained in the plasma membrane following primary granule exocytosis. In summary, these findings establish that triggering receptor expressed on myeloid cell-like transcript 2 expression is conserved between species and is likely to be important in regulating neutrophil antimicrobial function following primary granule exocytosis.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalJournal of Leukocyte Biology
Volume100
Issue number1
DOIs
StatePublished - Jul 2016

Keywords

  • Exocytosis
  • Leukocyte
  • TLT2

Fingerprint

Dive into the research topics of 'TREM-like transcript 2 is stored in human neutrophil primary granules and is up-regulated in response to inflammatory mediators'. Together they form a unique fingerprint.

Cite this