TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria

Elsa Noah N'Diaye, Catherine S. Branda, Steven S. Branda, Lisette Nevarez, Marco Colonna, Clifford Lowell, Jessica A. Hamerman, William E. Seaman

Research output: Contribution to journalArticlepeer-review

143 Scopus citations

Abstract

Phagocytosis, which is essential for the immune response to pathogens, is initiated by specifi c interactions between pathogens and cell surface receptors expressed by phagocytes. This study identifi es triggering receptor expressed on myeloid cells 2 (TREM-2) and its signaling counterpart DAP12 as a molecular complex that promotes phagocytosis of bacteria. Expression of TREM-2 - DAP12 enables nonphagocytic Chinese hamster ovary cells to internalize bacteria. This function depends on actin cytoskeleton dynamics and the activity of the small guanosine triphosphatases Rac and Cdc42. Internalization also requires src kinase activity and tyrosine phosphorylation. In bone marrow - derived macrophages, phagocytosis is decreased in the absence of DAP12 and can be restored by expression of TREM-2 - DAP12. Depletion of TREM-2 inhibits both binding and uptake of bacteria. Finally, TREM-2 - dependent phagocytosis is impaired in Syk-defi cient macrophages. This study highlights a novel role for TREM-2 - DAP12 in the immune response to bacterial pathogens.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalJournal of Cell Biology
Volume184
Issue number2
DOIs
StatePublished - Jan 26 2009

Fingerprint Dive into the research topics of 'TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria'. Together they form a unique fingerprint.

Cite this