TY - JOUR
T1 - TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria
AU - N'Diaye, Elsa Noah
AU - Branda, Catherine S.
AU - Branda, Steven S.
AU - Nevarez, Lisette
AU - Colonna, Marco
AU - Lowell, Clifford
AU - Hamerman, Jessica A.
AU - Seaman, William E.
PY - 2009/1/26
Y1 - 2009/1/26
N2 - Phagocytosis, which is essential for the immune response to pathogens, is initiated by specifi c interactions between pathogens and cell surface receptors expressed by phagocytes. This study identifi es triggering receptor expressed on myeloid cells 2 (TREM-2) and its signaling counterpart DAP12 as a molecular complex that promotes phagocytosis of bacteria. Expression of TREM-2 - DAP12 enables nonphagocytic Chinese hamster ovary cells to internalize bacteria. This function depends on actin cytoskeleton dynamics and the activity of the small guanosine triphosphatases Rac and Cdc42. Internalization also requires src kinase activity and tyrosine phosphorylation. In bone marrow - derived macrophages, phagocytosis is decreased in the absence of DAP12 and can be restored by expression of TREM-2 - DAP12. Depletion of TREM-2 inhibits both binding and uptake of bacteria. Finally, TREM-2 - dependent phagocytosis is impaired in Syk-defi cient macrophages. This study highlights a novel role for TREM-2 - DAP12 in the immune response to bacterial pathogens.
AB - Phagocytosis, which is essential for the immune response to pathogens, is initiated by specifi c interactions between pathogens and cell surface receptors expressed by phagocytes. This study identifi es triggering receptor expressed on myeloid cells 2 (TREM-2) and its signaling counterpart DAP12 as a molecular complex that promotes phagocytosis of bacteria. Expression of TREM-2 - DAP12 enables nonphagocytic Chinese hamster ovary cells to internalize bacteria. This function depends on actin cytoskeleton dynamics and the activity of the small guanosine triphosphatases Rac and Cdc42. Internalization also requires src kinase activity and tyrosine phosphorylation. In bone marrow - derived macrophages, phagocytosis is decreased in the absence of DAP12 and can be restored by expression of TREM-2 - DAP12. Depletion of TREM-2 inhibits both binding and uptake of bacteria. Finally, TREM-2 - dependent phagocytosis is impaired in Syk-defi cient macrophages. This study highlights a novel role for TREM-2 - DAP12 in the immune response to bacterial pathogens.
UR - http://www.scopus.com/inward/record.url?scp=60849109512&partnerID=8YFLogxK
U2 - 10.1083/jcb.200808080
DO - 10.1083/jcb.200808080
M3 - Article
C2 - 19171755
AN - SCOPUS:60849109512
SN - 0021-9525
VL - 184
SP - 215
EP - 223
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 2
ER -