Transplantable sites confer calcium sensitivity to BK channels

Matthew Schreiber, Alex Yuan, Lawrence Salkoff

Research output: Contribution to journalArticlepeer-review

123 Scopus citations


Both intracellular calcium and voltage activate Slo1, a high-conductance potassium channel, linking calcium with electrical excitability. Using molecular techniques, we created a calcium-insensitive variant of this channel gated by voltage alone. Calcium sensitivity was restored by adding back small portions of the carboxyl (C)-terminal 'tail' domain. Two separate regions of the tail independently conferred different degrees of calcium sensitivity; together, they restored essentially wild-type calcium dependence. These results suggest that, in the absence of calcium, the Slo1 tail inhibits voltage-dependent gating, and that calcium removes this inhibition. Slo1 may have evolved from an ancestral voltage-sensitive potassium channel represented by the core; the tail may represent the more recent addition of a calcium-dependent modulatory domain.

Original languageEnglish
Pages (from-to)416-421
Number of pages6
JournalNature neuroscience
Issue number5
StatePublished - May 1999


Dive into the research topics of 'Transplantable sites confer calcium sensitivity to BK channels'. Together they form a unique fingerprint.

Cite this