TY - JOUR
T1 - Transmission from the dominant input shapes the stereotypic ratio of photoreceptor inputs onto horizontal cells
AU - Yoshimatsu, Takeshi
AU - Williams, Philip R.
AU - D'Orazi, Florence D.
AU - Suzuki, Sachihiro C.
AU - Fadool, James M.
AU - Allison, W. Ted
AU - Raymond, Pamela A.
AU - Wong, Rachel O.
N1 - Funding Information:
We thank S. Kawamura for providing Tg(sws1:GFP) and Tg(sws2:GFP) transgenic fish, and D.R. Hyde and J. Nathans for providing ultraviolet and blue opsin antibodies. We also thank Wong lab members for helpful discussions and critical reading of the manuscript. This study is supported by NIH grants EY14358 to R.O.W., EY015509 to P.A.R., EY017753 to J.M.F., the Vision Core Grant EY01730, the Vision Training Grant EY07031 and Developmental Biology Training Grant HD07183 to F.D.D., Natural Sciences and Engineering Research Council of Canada to W.T.A., Uehara Memorial Foundation to T.Y. and S.C.S.
PY - 2014/5/15
Y1 - 2014/5/15
N2 - Many neurons receive synapses in stereotypic proportions from converging but functionally distinct afferents. However, developmental mechanisms regulating synaptic convergence are not well understood. Here we describe a heterotypic mechanism by which one afferent controls synaptogenesis of another afferent, but not vice versa. Like other CNS circuits, zebrafish retinal H3 horizontal cells (HC) undergo an initial period of remodelling, establishing synapses with ultraviolet and blue cones while eliminating red and green cone contacts. As development progresses, the HCs selectively synapse with ultraviolet cones to generate a 5:1 ultraviolet-to-blue cone synapse ratio. Blue cone synaptogenesis increases in mutants lacking ultraviolet cones, and when transmitter release or visual stimulation of ultraviolet cones is perturbed. Connectivity is unaltered when blue cone transmission is suppressed. Moreover, there is no cell-autonomous regulation of cone synaptogenesis by neurotransmission. Thus, biased connectivity in this circuit is established by an unusual activity-dependent, unidirectional control of synaptogenesis exerted by the dominant input.
AB - Many neurons receive synapses in stereotypic proportions from converging but functionally distinct afferents. However, developmental mechanisms regulating synaptic convergence are not well understood. Here we describe a heterotypic mechanism by which one afferent controls synaptogenesis of another afferent, but not vice versa. Like other CNS circuits, zebrafish retinal H3 horizontal cells (HC) undergo an initial period of remodelling, establishing synapses with ultraviolet and blue cones while eliminating red and green cone contacts. As development progresses, the HCs selectively synapse with ultraviolet cones to generate a 5:1 ultraviolet-to-blue cone synapse ratio. Blue cone synaptogenesis increases in mutants lacking ultraviolet cones, and when transmitter release or visual stimulation of ultraviolet cones is perturbed. Connectivity is unaltered when blue cone transmission is suppressed. Moreover, there is no cell-autonomous regulation of cone synaptogenesis by neurotransmission. Thus, biased connectivity in this circuit is established by an unusual activity-dependent, unidirectional control of synaptogenesis exerted by the dominant input.
UR - http://www.scopus.com/inward/record.url?scp=84900874512&partnerID=8YFLogxK
U2 - 10.1038/ncomms4699
DO - 10.1038/ncomms4699
M3 - Article
C2 - 24832361
AN - SCOPUS:84900874512
SN - 2041-1723
VL - 5
JO - Nature communications
JF - Nature communications
M1 - 3699
ER -