TY - JOUR
T1 - Translationsl regulation of expression of the bacteriophage T4 lysozyme gene
AU - Mcpheeters, David S.
AU - Christensen, Alan
AU - Young, Elton T.
AU - Stormo, Gary
AU - Gold, Larry
N1 - Funding Information:
ACKNOWLEDGEMENTS We are grateful to Gerry Smith, Ronald Wetzel, Jeanne Perry and Herbert Heyneker for communicating results before publication; Tan Inoue and Thomas Schneider for help with experiments; and Michael Parker, Duane Mooney, and Gus Doermann for helpful discussions. The lysozyme primers were made by Margit Gayle and Roger Saunders. This work was supported by Public Health Service Grants AI-09456, GM28755, and GM19963 from the National Institutes of Health.
PY - 1986/7/11
Y1 - 1986/7/11
N2 - The bacteriophage T4 lysozyme gene is transcribed at early and late times after infection of E. coli, but the early mRNA is not translated. DNA sequence analysis and mapping of the 5′ ends of the lysozyme transcripts produced at different times after T4 infection show that the early mRNA is Initiated some distance upstream from the gene. The early mRNA is not translated because of a stable secondary structure which blocks the translational initiation site. The stable RNA structure has been demonstrated by nudease protection in vivo After DNA replication begins, two late promoters are activated; the late transcripts are initiated at sites such that the secondary structure can not form, and translation of the late messages occurs.
AB - The bacteriophage T4 lysozyme gene is transcribed at early and late times after infection of E. coli, but the early mRNA is not translated. DNA sequence analysis and mapping of the 5′ ends of the lysozyme transcripts produced at different times after T4 infection show that the early mRNA is Initiated some distance upstream from the gene. The early mRNA is not translated because of a stable secondary structure which blocks the translational initiation site. The stable RNA structure has been demonstrated by nudease protection in vivo After DNA replication begins, two late promoters are activated; the late transcripts are initiated at sites such that the secondary structure can not form, and translation of the late messages occurs.
UR - http://www.scopus.com/inward/record.url?scp=0023056832&partnerID=8YFLogxK
U2 - 10.1093/nar/14.14.5813
DO - 10.1093/nar/14.14.5813
M3 - Article
C2 - 3526285
AN - SCOPUS:0023056832
SN - 0305-1048
VL - 14
SP - 5813
EP - 5826
JO - Nucleic acids research
JF - Nucleic acids research
IS - 14
ER -