TY - JOUR
T1 - Transition from cMyc to L-Myc during dendritic cell development coordinated by rising levels of IRF8
AU - Anderson, David A.
AU - Ou, Feiya
AU - Kim, Sunkyung
AU - Murphy, Theresa L.
AU - Murphy, Kenneth M.
N1 - Funding Information:
We thank the Genome Technology Access Center in the Department of Genetics at the Washington University School of Medicine for help with genomic analysis. The center is partially supported by the National Cancer Institute’s Cancer Center Support grant no. P30 CA91842 to the Siteman Cancer Center and by the National Center for Research Resources Institute of Clinical and Translational Sciences/Clinical and Translational Science Award grant no. UL1TR000448, a component of the National Institutes of Health and National Institutes of Health Roadmap for Medical Research. This publication is solely the responsibility of the authors and does not necessarily represent the official view of the National Center for Research Resources or National Institutes of Health. This work was supported by the National Institutes of Health (grant no. R01AI150297 to K.M. Murphy), the National Science Foundation (grant no. DGE-1143954 to D.A. Anderson), and the National Cancer Institute of the National Institutes of Health (grant nos. F31 CA228240 and T32 CA9547-35 to D.A. Anderson).
Publisher Copyright:
© 2021 Anderson et al.
PY - 2021/12/27
Y1 - 2021/12/27
N2 - During dendritic cell (DC) development, Myc expression in progenitors is replaced by Mycl in mature DCs, but when and how this transition occurs is unknown. We evaluated DC development using reporters for MYC, MYCL, and cell cycle proteins Geminin and CDT1 in wild-type and various mutant mice. For classical type 1 dendritic cells (cDC1s) and plasmacytoid DCs (pDCs), the transition occurred upon their initial specification from common dendritic cell progenitors (CDPs) or common lymphoid progenitors (CLPs), respectively. This transition required high levels of IRF8 and interaction with PU.1, suggesting the use of EICEs within Mycl enhancers. In pDCs, maximal MYCL induction also required the +41kb Irf8 enhancer that controls pDC IRF8 expression. IRF8 also contributed to repression of MYC. While MYC is expressed only in rapidly dividing DC progenitors, MYCL is most highly expressed in DCs that have exited the cell cycle. Thus, IRF8 levels coordinate the Myc-Mycl transition during DC development.
AB - During dendritic cell (DC) development, Myc expression in progenitors is replaced by Mycl in mature DCs, but when and how this transition occurs is unknown. We evaluated DC development using reporters for MYC, MYCL, and cell cycle proteins Geminin and CDT1 in wild-type and various mutant mice. For classical type 1 dendritic cells (cDC1s) and plasmacytoid DCs (pDCs), the transition occurred upon their initial specification from common dendritic cell progenitors (CDPs) or common lymphoid progenitors (CLPs), respectively. This transition required high levels of IRF8 and interaction with PU.1, suggesting the use of EICEs within Mycl enhancers. In pDCs, maximal MYCL induction also required the +41kb Irf8 enhancer that controls pDC IRF8 expression. IRF8 also contributed to repression of MYC. While MYC is expressed only in rapidly dividing DC progenitors, MYCL is most highly expressed in DCs that have exited the cell cycle. Thus, IRF8 levels coordinate the Myc-Mycl transition during DC development.
UR - http://www.scopus.com/inward/record.url?scp=85122706282&partnerID=8YFLogxK
U2 - 10.1084/jem.20211483
DO - 10.1084/jem.20211483
M3 - Article
C2 - 34958351
AN - SCOPUS:85122706282
SN - 0022-1007
VL - 219
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 2
M1 - e20211483
ER -