Transient localized accumulation of actin in Caenorhabditis elegans blastomeres with oriented asymmetric divisions

James A. Waddle, John A. Cooper, Robert H. Waterston

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

During Caenorhabditis elegans embryogenesis, specific cells in the P1 lineage rotate their duplicated centrosome pair onto the anterior-posterior axis; this rotation is correlated with and necessary for a differential inheritance of cytoplasmic determinants in the daughter cells. Centrosome pair rotation is sensitive to inhibitors of actin and microtubule polymerization and may require microtubule attachment to a specific cortical site. We show that actin and the barbed-end binding protein, capping protein, transiently accumulate at this cortical site, possibly by assembly onto persistent remnants of previous cell divisions. Based on these observations, we propose a model for the molecular basis of centrosome rotation that is consistent with the dependence of rotation on actin filaments and microtubules.

Original languageEnglish
Pages (from-to)2317-2328
Number of pages12
JournalDevelopment
Volume120
Issue number8
StatePublished - Aug 1 1994

Keywords

  • Actin
  • Asymmetric division
  • Caenorhabditis embryogenesis
  • Cell division
  • Centrosome

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