Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

Bin Yu; Elisabeth J. Chapman; Zhiyong Yang; James C. Carrington; Xuemei Chen

doi:10.1016/j.febslet.2006.04.063

Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

Bin Yu
, Elisabeth J. Chapman
, Zhiyong Yang
, James C. Carrington
, Xuemei Chen

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA^* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA^* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA^* duplexes in vivo. These findings suggest miRNA/miRNA^* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.

Original language	English
Pages (from-to)	3117-3120
Number of pages	4
Journal	FEBS Letters
Volume	580
Issue number	13
DOIs	https://doi.org/10.1016/j.febslet.2006.04.063
State	Published - May 29 2006

Keywords

HEN1
Methylation
Viral RNA silencing suppressor
microRNA/microRNA

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10.1016/j.febslet.2006.04.063

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title = "Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis",

abstract = "HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA* duplexes in vivo. These findings suggest miRNA/miRNA* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.",

keywords = "HEN1, Methylation, Viral RNA silencing suppressor, microRNA/microRNA",

author = "Bin Yu and Chapman, \{Elisabeth J.\} and Zhiyong Yang and Carrington, \{James C.\} and Xuemei Chen",

note = "Funding Information: This work was supported by NSF (MCB-0612958 to X.C. and MCB-0209836 to J.C.). ",

year = "2006",

month = may,

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doi = "10.1016/j.febslet.2006.04.063",

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TY - JOUR

T1 - Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

AU - Yu, Bin

AU - Chapman, Elisabeth J.

AU - Yang, Zhiyong

AU - Carrington, James C.

AU - Chen, Xuemei

N1 - Funding Information: This work was supported by NSF (MCB-0612958 to X.C. and MCB-0209836 to J.C.).

PY - 2006/5/29

Y1 - 2006/5/29

N2 - HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA* duplexes in vivo. These findings suggest miRNA/miRNA* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.

AB - HEN1-dependent methylation of the 3′-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA* duplexes in vivo. These findings suggest miRNA/miRNA* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation.

KW - HEN1

KW - Methylation

KW - Viral RNA silencing suppressor

KW - microRNA/microRNA

UR - https://www.scopus.com/pages/publications/33646589610

U2 - 10.1016/j.febslet.2006.04.063

DO - 10.1016/j.febslet.2006.04.063

M3 - Article

C2 - 16678167

AN - SCOPUS:33646589610

SN - 0014-5793

VL - 580

SP - 3117

EP - 3120

JO - FEBS Letters

JF - FEBS Letters

IS - 13

ER -

Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

Abstract

Keywords

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