TY - JOUR
T1 - Transfusion-related Epstein-Barr virus (EBV) infection
T2 - A multicenter prospective cohort study among pediatric recipients of hematopoietic stem cell transplants (TREASuRE study)
AU - Enok Bonong, Pascal R.
AU - Buteau, Chantal
AU - Delage, Gilles
AU - Tanner, Jerome E.
AU - Lacroix, Jacques
AU - Duval, Michel
AU - Laporte, Louise
AU - Tucci, Marisa
AU - Robitaille, Nancy
AU - Spinella, Philip C.
AU - Cuvelier, Geoffrey
AU - Vercauteren, Suzanne
AU - Lewis, Victor
AU - Fearon, Margaret
AU - Drews, Steven J.
AU - Alfieri, Carolina
AU - Trottier, Helen
N1 - Funding Information:
The authors are grateful to Héma-Québec and Canadian Blood Services. The authors graciously thank Nicole Poitras, Lucy Clayton, Ferima Sanogo, Caroline Proulx-Clerc, Isabelle Grisoni, Ilona Shemyakina, Sylvie Lacasse for coordinating the TREASuRE study; Mary-Ellen French, Djouher Nait Ladjemil, Mariana Dumitrascu, Emilie Roy-Robertson, Gaëlle Cyr, Maria Trinidad Madrid Guillen for collecting data. All authors are also grateful to the transplant team: Marie-France Vachon, Marie St-Jacques, Samira Mezziani, Marion Cortier, and all nurses. Our thanks to Sylvie Tremblay and Sylvie Lebel at Héma-Québec for managing blood donor participants, to Irene Dines from CBS; to HSCT recipients and blood donors who consented to provide blood samples for EBV genotyping analysis; and to Jing Hu for technical assistance with blood separation and processing, DNA extraction, LCL generation, and preparation of samples for sequencing. TREASuRE study was supported by a grant from Canadian Blood Services (CBS). HT holds a salary award (Research Scholar) from the Fonds de la recherche du Québec en santé (FRQ-S) and a new Investigator Salary Award from the Canadian Institutes of Health Research (CIHR).
Funding Information:
The authors are grateful to Héma‐Québec and Canadian Blood Services. The authors graciously thank Nicole Poitras, Lucy Clayton, Ferima Sanogo, Caroline Proulx‐Clerc, Isabelle Grisoni, Ilona Shemyakina, Sylvie Lacasse for coordinating the TREASuRE study; Mary‐Ellen French, Djouher Nait Ladjemil, Mariana Dumitrascu, Emilie Roy‐Robertson, Gaëlle Cyr, Maria Trinidad Madrid Guillen for collecting data. All authors are also grateful to the transplant team: Marie‐France Vachon, Marie St‐Jacques, Samira Mezziani, Marion Cortier, and all nurses. Our thanks to Sylvie Tremblay and Sylvie Lebel at Héma‐Québec for managing blood donor participants, to Irene Dines from CBS; to HSCT recipients and blood donors who consented to provide blood samples for EBV genotyping analysis; and to Jing Hu for technical assistance with blood separation and processing, DNA extraction, LCL generation, and preparation of samples for sequencing. TREASuRE study was supported by a grant from Canadian Blood Services (CBS). HT holds a salary award (Research Scholar) from the Fonds de la recherche du Québec en santé (FRQ‐S) and a new Investigator Salary Award from the Canadian Institutes of Health Research (CIHR).
Publisher Copyright:
© 2020 AABB
PY - 2021/1
Y1 - 2021/1
N2 - Background: Epstein-Barr virus (EBV) is carried in the blood of most adults, and transfusion-related infections have been reported. EBV is particularly deleterious in immunosuppressed transplant patients. The aim was to determine if EBV transmission occurred through leukodepleted blood product transfusion in pediatric recipients of hematopoietic stem cell transplants (HSCT). Study Design and Methods: This prospective Canadian multi-center cohort study includes 156 allogeneic HSCT pediatric recipients. The association between EBV and transfusion was analyzed using Cox regressions. EBV infection, defined by a PCR+ test in the blood of seronegative recipients of an EBV-negative graft, was monitored in order to correlate the recipient EBV strain with that of the blood donors. EBV genotypes were determined by PCR amplification followed by DNA sequencing at two loci (EBNA3b and LMP1). Results: No statistically significant associations were found between transfusions and EBV. One case of post-transplant EBV infection was identified among the 21 EBV-seronegative recipients receiving an EBV-negative graft. A total of 22 blood donors were retraced to determine whether the recipientʼs EBV strain matched that of a donor. One donor strain showed 100% sequence homology at the EBNA3b locus, but differed by one or two point mutations and by a 132-bp deletion at the LMP1 locus. The blood donor in question was alone among the 22 donors to show amplifiable virus in plasma. Blood from this donor readily produced an immortalized lymphoblastoid cell line in culture. Conclusion: While considered a rare event, EBV transmission through transfusion may occur in the context of severe immunosuppression.
AB - Background: Epstein-Barr virus (EBV) is carried in the blood of most adults, and transfusion-related infections have been reported. EBV is particularly deleterious in immunosuppressed transplant patients. The aim was to determine if EBV transmission occurred through leukodepleted blood product transfusion in pediatric recipients of hematopoietic stem cell transplants (HSCT). Study Design and Methods: This prospective Canadian multi-center cohort study includes 156 allogeneic HSCT pediatric recipients. The association between EBV and transfusion was analyzed using Cox regressions. EBV infection, defined by a PCR+ test in the blood of seronegative recipients of an EBV-negative graft, was monitored in order to correlate the recipient EBV strain with that of the blood donors. EBV genotypes were determined by PCR amplification followed by DNA sequencing at two loci (EBNA3b and LMP1). Results: No statistically significant associations were found between transfusions and EBV. One case of post-transplant EBV infection was identified among the 21 EBV-seronegative recipients receiving an EBV-negative graft. A total of 22 blood donors were retraced to determine whether the recipientʼs EBV strain matched that of a donor. One donor strain showed 100% sequence homology at the EBNA3b locus, but differed by one or two point mutations and by a 132-bp deletion at the LMP1 locus. The blood donor in question was alone among the 22 donors to show amplifiable virus in plasma. Blood from this donor readily produced an immortalized lymphoblastoid cell line in culture. Conclusion: While considered a rare event, EBV transmission through transfusion may occur in the context of severe immunosuppression.
UR - http://www.scopus.com/inward/record.url?scp=85093508003&partnerID=8YFLogxK
U2 - 10.1111/trf.16149
DO - 10.1111/trf.16149
M3 - Article
C2 - 33089891
AN - SCOPUS:85093508003
SN - 0041-1132
VL - 61
SP - 144
EP - 158
JO - Transfusion
JF - Transfusion
IS - 1
ER -