Transforming growth factor beta-1 and beta-2 in human tear fluid

Anurag Gupta, Dagoberto Monroy, Zhonghua Ji, Kenichi Yoshino, Andrew Huang, Stephen C. Pflugfelder

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

Purpose. To evaluate human tear fluid for transforming growth factor beta isoforms 1 and 2 (TGF-β1 and TGF-β2). Methods. To accomplish this, human tears were evaluated for TGF-βs by quantitative antibody sandwich ELISA (sELISA), mink lung epithelial cell (MLEC) growth inhibition bioassay and western blotting. Various physical and chemical treatments were used to activate TGF-β in these assays. Results. TGF-βs could not be detected in untreated or heated tears by sELISA; however, mean TGF-β1 concentrations of 38.5 ng/ml and TGF-β2 concentrations of 2.32 ng/ml were detected in acid-activated tears by sELISA. Furthermore, 10.54 ng/ml of TGF-β1 and 3.98 ng/ml of TCF-β2 were detected in tears treated with the mucolytic agent, acetylcysteine. Total TGF-β bioactivity in human tears measured by the MLEC assay was found to be 13.04 ng/ml in untreated tears and 24.85 ng/ml in acid-activated tears. Approximately one-half TGF-β in tear specimens was biologically active (mean = 52%, range 39-71%). Total tear TGF-β bioactivity could be completely neutralized by recombinant human TGF-β1 latency associated peptide (rh TGF-β1 LAP). Mean neutralization of tear TGF-β bioactivity was 83% by TGF-β1-specific antisera, and was 13% by TGF-β2-specific antisera. Immunoreactive TGF-β bands at approximately 12.5 and 95 kD were observed in immunoblots of reduced acidified tears. A high molecular weight (MW) TGF-β band (> 203 kD) was noted in untreated tears; however, this band disappeared following treatment with acetylcysteine. Conclusions. The results of these studies indicate that TGF-β 1 and TGF-β2 are present in human tear fluid, and TGF-β1 is the predominant isoform. There appears to be factors in human tears capable of binding TGF-β.

Original languageEnglish
Pages (from-to)605-614
Number of pages10
JournalCurrent Eye Research
Volume15
Issue number6
DOIs
StatePublished - 1996

Keywords

  • Human
  • TGF-β1
  • TGF-β2
  • Tear proteins
  • Tears
  • Transforming growth factor beta (TGF-β)

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