Transformation of yeast spheroplasts without cell fusion

Peter M.J. Burgers; Kimberly J. Percival

doi:10.1016/0003-2697(87)90240-5

Transformation of yeast spheroplasts without cell fusion

Peter M.J. Burgers
, Kimberly J. Percival

Research output: Contribution to journal › Article › peer-review

248 Scopus citations

Abstract

The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 × 10⁷ and 5 × 10⁶ transformants/μg, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.

Original language	English
Pages (from-to)	391-397
Number of pages	7
Journal	Analytical Biochemistry
Volume	163
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(87)90240-5
State	Published - Jun 1987

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@article{c9adb25ae99d4900b5a55de8ac332857,

title = "Transformation of yeast spheroplasts without cell fusion",

abstract = "The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 × 107 and 5 × 106 transformants/μg, respectively. At saturating DNA concentrations, 12 and 3\%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3\%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.",

author = "Burgers, \{Peter M.J.\} and Percival, \{Kimberly J.\}",

note = "Funding Information: This work was supported by Public Health Service Grant GM 3243 1 from the National Institutes of Health and by Award 83-A-105 from the Chicago Community Trust/Searle Scholars Program (to P.M.J.B.).",

year = "1987",

month = jun,

doi = "10.1016/0003-2697(87)90240-5",

language = "English",

volume = "163",

pages = "391--397",

journal = "Analytical Biochemistry",

issn = "0003-2697",

number = "2",

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T1 - Transformation of yeast spheroplasts without cell fusion

AU - Burgers, Peter M.J.

AU - Percival, Kimberly J.

N1 - Funding Information: This work was supported by Public Health Service Grant GM 3243 1 from the National Institutes of Health and by Award 83-A-105 from the Chicago Community Trust/Searle Scholars Program (to P.M.J.B.).

PY - 1987/6

Y1 - 1987/6

N2 - The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 × 107 and 5 × 106 transformants/μg, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.

AB - The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 × 107 and 5 × 106 transformants/μg, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.

UR - https://www.scopus.com/pages/publications/0023360013

U2 - 10.1016/0003-2697(87)90240-5

DO - 10.1016/0003-2697(87)90240-5

M3 - Article

C2 - 3310730

AN - SCOPUS:0023360013

SN - 0003-2697

VL - 163

SP - 391

EP - 397

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Transformation of yeast spheroplasts without cell fusion

Abstract

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