TY - JOUR
T1 - Transformation of yeast spheroplasts without cell fusion
AU - Burgers, Peter M.J.
AU - Percival, Kimberly J.
N1 - Funding Information:
This work was supported by Public Health Service Grant GM 3243 1 from the National Institutes of Health and by Award 83-A-105 from the Chicago Community Trust/Searle Scholars Program (to P.M.J.B.).
PY - 1987/6
Y1 - 1987/6
N2 - The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 × 107 and 5 × 106 transformants/μg, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.
AB - The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 × 107 and 5 × 106 transformants/μg, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.
UR - http://www.scopus.com/inward/record.url?scp=0023360013&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(87)90240-5
DO - 10.1016/0003-2697(87)90240-5
M3 - Article
C2 - 3310730
AN - SCOPUS:0023360013
SN - 0003-2697
VL - 163
SP - 391
EP - 397
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -