Transformation of NIH 3T3 cells by a human c-sis cDNA clone

Michael F. Clarke, Eric Westin, David Schmidt, Steven F. Josephs, Lee Ratner, Flossie Wong-Staal, Robert C. Gallo, Marvin S. Reitz

Research output: Contribution to journalArticlepeer-review

131 Scopus citations

Abstract

The mechanism of leukaemogenk transformation by human T-cell leukaemia/Iymphoma virus (HTLV), a retrovirus implicated in the aetiology of certain adult T-cell leukaemias and lymphomas, is unknown but is conceivably associated with the expression of the cellular analogues of retroviral oncogenes. The HUT-102 cell line1, derived from a cutaneous T-cell lymphoma and infected with HTLV2-4, expresses several cellular oncogenes5. It is unusual among haemopoietic cell lines in that one of these is c-sis, the gene from which the oncogene v-sis of the simian sarcoma virus was derived, and perhaps the gene for platelet-derived growth factor (PDGF)6,7. To explore the possible role of c-sis expression in HTLV-induced disease, we have obtained cDNA clones of c-sis from HUT-102 cells. Here we describe two such clones and report that one of them transforms NIH-3T3 cells. This is the first example of transformation of NIH-3T3 cells by a human onc gene other than c-ras or Blym, as well as the first demonstration of transformation by a human cDNA clone.

Original languageEnglish
Pages (from-to)464-467
Number of pages4
JournalNature
Volume308
Issue number5958
DOIs
StatePublished - 1984

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