Transdermal fluorescence detection of a dual fluorophore system for noninvasive point-of-care gastrointestinal permeability measurement

Richard B. Dorshow, J. R. Johnson, Martin P. Debreczeny, I. Rochelle Riley, Jeng Jong Shieh, Thomas E. Rogers, Carla Hall-Moore, Nurmohammad Shaikh, L. Colleen Rouggly-Nickless, Phillip I. Tarr

Research output: Contribution to journalArticle

Abstract

The intestinal mucosal barrier prevents macromolecules and pathogens from entering the circulatory stream. Tight junctions in this barrier are compromised in inflammatory bowel diseases, environmental enteropathy, and enteric dysfunction. Dual sugar absorption tests are a standard method for measuring gastrointestinal integrity, however, these are not clinically amenable. Herein, we report on a dual fluorophore system and fluorescence detection instrumentation for which gastrointestinal permeability is determined in a rat small bowel disease model from the longitudinal measured transdermal fluorescence of each fluorophore. This fluorophore technology enables a specimen-free, noninvasive, point-of-care gastrointestinal permeability measurement which should be translatable to human clinical studies.

Original languageEnglish
Pages (from-to)5103-5116
Number of pages14
JournalBiomedical Optics Express
Volume10
Issue number10
DOIs
StatePublished - Oct 1 2019

Fingerprint Dive into the research topics of 'Transdermal fluorescence detection of a dual fluorophore system for noninvasive point-of-care gastrointestinal permeability measurement'. Together they form a unique fingerprint.

  • Cite this

    Dorshow, R. B., Johnson, J. R., Debreczeny, M. P., Riley, I. R., Shieh, J. J., Rogers, T. E., Hall-Moore, C., Shaikh, N., Rouggly-Nickless, L. C., & Tarr, P. I. (2019). Transdermal fluorescence detection of a dual fluorophore system for noninvasive point-of-care gastrointestinal permeability measurement. Biomedical Optics Express, 10(10), 5103-5116. https://doi.org/10.1364/BOE.10.005103