TY - JOUR
T1 - Transcriptional programs define molecular characteristics of innate lymphoid cell classes and subsets
AU - Robinette, Michelle L.
AU - Fuchs, Anja
AU - Cortez, Victor S.
AU - Lee, Jacob S.
AU - Wang, Yaming
AU - Durum, Scott K.
AU - Gilfillan, Susan
AU - Colonna, Marco
N1 - Funding Information:
We thank our colleagues in the ImmGen consortium, especially C. Benoist and L. Lanier, for input and discussion; the core ImmGen team, K. Rothamel and A. Rhodes, for contributions and technical assistance; M. Artyomov, G. Krishnan and J. Siegel for computational assistance; D. Sojka for discussion; E. Lantelme and D. Brinja for sorting assistance; P. Wang for microscopy assistance; and eBioscience and Affymetrix for support of the ImmGen Project. Supported by the US National Institutes of Health (R24AI072073 to the ImmGen Consortium; 1U01AI095542, R01DE021255 and R21CA16719 to the Colonna laboratory; MSTP T32 GM07200 to M.L.R.; and Infectious Disease Training Grant T32 AI 7172-34 to V.S.C.).
Publisher Copyright:
© 2015 Nature America, Inc.
PY - 2015/2/17
Y1 - 2015/2/17
N2 - The recognized diversity of innate lymphoid cells (ILCs) is rapidly expanding. Three ILC classes have emerged, ILC1, ILC2 and ILC3, with ILC1 and ILC3 including several subsets. The classification of some subsets is unclear, and it remains controversial whether natural killer (NK) cells and ILC1 cells are distinct cell types. To address these issues, we analyzed gene expression in ILCs and NK cells from mouse small intestine, spleen and liver, as part of the Immunological Genome Project. The results showed unique gene-expression patterns for some ILCs and overlapping patterns for ILC1 cells and NK cells, whereas other ILC subsets remained indistinguishable. We identified a transcriptional program shared by small intestine ILCs and a core ILC signature. We revealed and discuss transcripts that suggest previously unknown functions and developmental paths for ILCs.
AB - The recognized diversity of innate lymphoid cells (ILCs) is rapidly expanding. Three ILC classes have emerged, ILC1, ILC2 and ILC3, with ILC1 and ILC3 including several subsets. The classification of some subsets is unclear, and it remains controversial whether natural killer (NK) cells and ILC1 cells are distinct cell types. To address these issues, we analyzed gene expression in ILCs and NK cells from mouse small intestine, spleen and liver, as part of the Immunological Genome Project. The results showed unique gene-expression patterns for some ILCs and overlapping patterns for ILC1 cells and NK cells, whereas other ILC subsets remained indistinguishable. We identified a transcriptional program shared by small intestine ILCs and a core ILC signature. We revealed and discuss transcripts that suggest previously unknown functions and developmental paths for ILCs.
UR - http://www.scopus.com/inward/record.url?scp=84923436228&partnerID=8YFLogxK
U2 - 10.1038/ni.3094
DO - 10.1038/ni.3094
M3 - Article
C2 - 25621825
AN - SCOPUS:84923436228
SN - 1529-2908
VL - 16
SP - 306
EP - 317
JO - Nature immunology
JF - Nature immunology
IS - 3
ER -