Transcription initiation activity of adenovirus left-end sequence in adenovirus vectors with E1 deleted

Masato Yamamoto, Julia Davydova, Koichi Takayama, Ramon Alemany, David T. Curiel

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We analyzed the transcription initiation activity of the left-end sequence (first 342 bp) of the adenovirus genome in the context of an adenovirus vector with E1 deleted in in vitro and in vivo gene transfer models. While nucleotide sequences 1 to 190 and 1 to 342 showed strong activity in three out of three lung cancer cell lines, nucleotide sequence 1 to 103 showed limited activity in H358, cells which show characteristics of type 2 alveolar cells. In vivo, the transcription initiation activities of nucleotide sequence 1 to 103 in the liver and the lung were minimal, while nucleotide sequences 1 to 190 and 1 to 342 showed strong activity comparable to that of the cytomegalovirus promoter. Further understanding of the transcription initiation activity of the left-end sequence of the adenovirus genome should lead to optimization of adenovirus vectors.

Original languageEnglish
Pages (from-to)1633-1637
Number of pages5
JournalJournal of virology
Volume77
Issue number2
DOIs
StatePublished - Jan 2003

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