TY - JOUR
T1 - Transcription factor NF-κB regulates inducible Oct-2 gene expression in precursor B lymphocytes
AU - Bendall, Heather H.
AU - Scherert, David C.
AU - Edson, Christine R.
AU - Ballard, Dean W.
AU - Oltz, Eugene M.
PY - 1997/11/14
Y1 - 1997/11/14
N2 - The POU transcription factors Oct-1 and Oct-2 regulate the activity of octamer-dependent promoters, including those that direct transcription from rearranged immunoglobulin genes. Unlike Oct-l, which is constitutively expressed in many cell types, Oct-2 expression is restricted primarily to B lymphocytes and can be induced in precursor B cells by stimulation with bacterial lipopolysaccharide (LPS). However, the precise factors that mediate this induction mechanism remain unknown. In the present study, we monitored Oct-2 expression in cells arrested for the activation of NF-κB, an LPS- responsive member of the Rel transcription factor family. Despite stimulation with LPS, disruption of the NF-κB signaling pathway in precursor B cells led to the loss of inducible Oct-2 DNA binding activity in vitro and the suppression of Oct-2-directed transcription in vivo. This biochemical defect correlated with a specific block to Oct-2 gene expression at the level of transcription, whereas the expression of Oct-1 was unaffected. The finding that Oct-2 is under NF-κB control highlights an important cross-talk mechanism involving two distinct transcription factor families that regulate B lymphocyte function.
AB - The POU transcription factors Oct-1 and Oct-2 regulate the activity of octamer-dependent promoters, including those that direct transcription from rearranged immunoglobulin genes. Unlike Oct-l, which is constitutively expressed in many cell types, Oct-2 expression is restricted primarily to B lymphocytes and can be induced in precursor B cells by stimulation with bacterial lipopolysaccharide (LPS). However, the precise factors that mediate this induction mechanism remain unknown. In the present study, we monitored Oct-2 expression in cells arrested for the activation of NF-κB, an LPS- responsive member of the Rel transcription factor family. Despite stimulation with LPS, disruption of the NF-κB signaling pathway in precursor B cells led to the loss of inducible Oct-2 DNA binding activity in vitro and the suppression of Oct-2-directed transcription in vivo. This biochemical defect correlated with a specific block to Oct-2 gene expression at the level of transcription, whereas the expression of Oct-1 was unaffected. The finding that Oct-2 is under NF-κB control highlights an important cross-talk mechanism involving two distinct transcription factor families that regulate B lymphocyte function.
UR - http://www.scopus.com/inward/record.url?scp=0030724751&partnerID=8YFLogxK
U2 - 10.1074/jbc.272.46.28826
DO - 10.1074/jbc.272.46.28826
M3 - Article
C2 - 9360945
AN - SCOPUS:0030724751
SN - 0021-9258
VL - 272
SP - 28826
EP - 28828
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 46
ER -