Transcription factor NF-κB regulates inducible Oct-2 gene expression in precursor B lymphocytes

Heather H. Bendall, David C. Scherert, Christine R. Edson, Dean W. Ballard, Eugene M. Oltz

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The POU transcription factors Oct-1 and Oct-2 regulate the activity of octamer-dependent promoters, including those that direct transcription from rearranged immunoglobulin genes. Unlike Oct-l, which is constitutively expressed in many cell types, Oct-2 expression is restricted primarily to B lymphocytes and can be induced in precursor B cells by stimulation with bacterial lipopolysaccharide (LPS). However, the precise factors that mediate this induction mechanism remain unknown. In the present study, we monitored Oct-2 expression in cells arrested for the activation of NF-κB, an LPS- responsive member of the Rel transcription factor family. Despite stimulation with LPS, disruption of the NF-κB signaling pathway in precursor B cells led to the loss of inducible Oct-2 DNA binding activity in vitro and the suppression of Oct-2-directed transcription in vivo. This biochemical defect correlated with a specific block to Oct-2 gene expression at the level of transcription, whereas the expression of Oct-1 was unaffected. The finding that Oct-2 is under NF-κB control highlights an important cross-talk mechanism involving two distinct transcription factor families that regulate B lymphocyte function.

Original languageEnglish
Pages (from-to)28826-28828
Number of pages3
JournalJournal of Biological Chemistry
Volume272
Issue number46
DOIs
StatePublished - Nov 14 1997

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