Transcription factor GATA-6 is expressed in the endocrine and GATA-4 in the exocrine pancreas

Ilkka Ketola, Timo Otonkoski, Mari Anne Pulkkinen, Hannele Niemi, Jaan Palgi, Christina M. Jacobsen, David B. Wilson, Markku Heikinheimo

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

GATA-4 and GATA-6 are zinc finger transcription factors that regulate gene expression, differentiation, and cell proliferation in various tissues. These factors have been implicated in the development of endodermal derivatives, including epithelial cells in the yolk sac, lung, and stomach. In the present study, we have characterized the expression of GATA-4 and GATA-6 during development of another endodermal derivative, the mouse pancreas, using a combination of in situ hybridization and immunohistochemistry. Neither GATA-4 nor GATA-6 antigen was detected in E10.5 pancreatic epithelial buds expressing Pdx-1. By E15.5, GATA-4 mRNA and protein were evident in developing pancreatic acini, but not in ductal or endocrine cells of the pancreas; GATA-6 mRNA and protein were present in both endocrine and exocrine cell precursors. In the newborn and adult pancreas, GATA-4 protein was seen in acinar cells, while GATA-6 antigen was found mainly in islet beta-cells. The amphicrine pancreatic AR42J-B13 cell line was used to study the expression of GATA-4 and GATA-6 during the differentiation of these cells towards an endocrine phenotype. Endocrine differentiation was associated with marked increase in GATA-6 but not GATA-4 mRNA levels. We conclude that GATA-4 is a marker of exocrine pancreatic differentiation, whereas GATA-6 is a marker of endocrine pancreatic development.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume226
Issue number1-2
DOIs
StatePublished - Oct 29 2004
Externally publishedYes

Keywords

  • Endocrine
  • Exocrine
  • GATA-4
  • GATA-6
  • Pancreas
  • Transcription factor

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    Ketola, I., Otonkoski, T., Pulkkinen, M. A., Niemi, H., Palgi, J., Jacobsen, C. M., Wilson, D. B., & Heikinheimo, M. (2004). Transcription factor GATA-6 is expressed in the endocrine and GATA-4 in the exocrine pancreas. Molecular and Cellular Endocrinology, 226(1-2), 51-57. https://doi.org/10.1016/j.mce.2004.06.007