TY - JOUR
T1 - Transcription factor GATA-6 is expressed in the endocrine and GATA-4 in the exocrine pancreas
AU - Ketola, Ilkka
AU - Otonkoski, Timo
AU - Pulkkinen, Mari Anne
AU - Niemi, Hannele
AU - Palgi, Jaan
AU - Jacobsen, Christina M.
AU - Wilson, David B.
AU - Heikinheimo, Markku
N1 - Funding Information:
This work was supported by, the Finnish Pediatric Research Foundation (to I.K. and M.H.), the Maud Kuistila Foundation (to I.K.), the Ahokas Foundation (to M.H.), the University Central Hospital in Helsinki (to I.K, M.-A.P., T.O., and M.H.), the Sigrid Juselius Foundation (to M.H., T.O, and D.B.W.), NIH grants HL61006 DK 52574 and the DRTC (to D.B.W.), and the Juvenile Diabetes Research Foundation International (to T.O.). We thank Päivi Kinnunen and Jarkko Ustinov for help.
PY - 2004/10/29
Y1 - 2004/10/29
N2 - GATA-4 and GATA-6 are zinc finger transcription factors that regulate gene expression, differentiation, and cell proliferation in various tissues. These factors have been implicated in the development of endodermal derivatives, including epithelial cells in the yolk sac, lung, and stomach. In the present study, we have characterized the expression of GATA-4 and GATA-6 during development of another endodermal derivative, the mouse pancreas, using a combination of in situ hybridization and immunohistochemistry. Neither GATA-4 nor GATA-6 antigen was detected in E10.5 pancreatic epithelial buds expressing Pdx-1. By E15.5, GATA-4 mRNA and protein were evident in developing pancreatic acini, but not in ductal or endocrine cells of the pancreas; GATA-6 mRNA and protein were present in both endocrine and exocrine cell precursors. In the newborn and adult pancreas, GATA-4 protein was seen in acinar cells, while GATA-6 antigen was found mainly in islet beta-cells. The amphicrine pancreatic AR42J-B13 cell line was used to study the expression of GATA-4 and GATA-6 during the differentiation of these cells towards an endocrine phenotype. Endocrine differentiation was associated with marked increase in GATA-6 but not GATA-4 mRNA levels. We conclude that GATA-4 is a marker of exocrine pancreatic differentiation, whereas GATA-6 is a marker of endocrine pancreatic development.
AB - GATA-4 and GATA-6 are zinc finger transcription factors that regulate gene expression, differentiation, and cell proliferation in various tissues. These factors have been implicated in the development of endodermal derivatives, including epithelial cells in the yolk sac, lung, and stomach. In the present study, we have characterized the expression of GATA-4 and GATA-6 during development of another endodermal derivative, the mouse pancreas, using a combination of in situ hybridization and immunohistochemistry. Neither GATA-4 nor GATA-6 antigen was detected in E10.5 pancreatic epithelial buds expressing Pdx-1. By E15.5, GATA-4 mRNA and protein were evident in developing pancreatic acini, but not in ductal or endocrine cells of the pancreas; GATA-6 mRNA and protein were present in both endocrine and exocrine cell precursors. In the newborn and adult pancreas, GATA-4 protein was seen in acinar cells, while GATA-6 antigen was found mainly in islet beta-cells. The amphicrine pancreatic AR42J-B13 cell line was used to study the expression of GATA-4 and GATA-6 during the differentiation of these cells towards an endocrine phenotype. Endocrine differentiation was associated with marked increase in GATA-6 but not GATA-4 mRNA levels. We conclude that GATA-4 is a marker of exocrine pancreatic differentiation, whereas GATA-6 is a marker of endocrine pancreatic development.
KW - Endocrine
KW - Exocrine
KW - GATA-4
KW - GATA-6
KW - Pancreas
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=5644301019&partnerID=8YFLogxK
U2 - 10.1016/j.mce.2004.06.007
DO - 10.1016/j.mce.2004.06.007
M3 - Article
C2 - 15489005
AN - SCOPUS:5644301019
SN - 0303-7207
VL - 226
SP - 51
EP - 57
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-2
ER -