TY - JOUR
T1 - Transcription and replication initiate at separate sites on the vesicular stomatitis virus genome
AU - Whelan, Sean P.J.
AU - Wertz, Gail W.
PY - 2002/7/9
Y1 - 2002/7/9
N2 - The RNA-dependent RNA polymerase of the nonsegmented negative-strand RNA viruses carries out two distinct RNA synthetic processes: Transcription of monocistronic, capped, and polyadenylated subgenomic messenger RNAs, and replication by means of the synthesis of a full-length positive-sense copy of the genome. The template for both processes is the negative-sense genomic RNA tightly encapsidated by the viral nucleocapsid protein. By applying UV transcriptional mapping to engineered variants of vesicular stomatitis virus, we discovered that, in infected cells, transcription and replication are controlled by initiation at different positions on the viral genome.
AB - The RNA-dependent RNA polymerase of the nonsegmented negative-strand RNA viruses carries out two distinct RNA synthetic processes: Transcription of monocistronic, capped, and polyadenylated subgenomic messenger RNAs, and replication by means of the synthesis of a full-length positive-sense copy of the genome. The template for both processes is the negative-sense genomic RNA tightly encapsidated by the viral nucleocapsid protein. By applying UV transcriptional mapping to engineered variants of vesicular stomatitis virus, we discovered that, in infected cells, transcription and replication are controlled by initiation at different positions on the viral genome.
UR - http://www.scopus.com/inward/record.url?scp=0037047108&partnerID=8YFLogxK
U2 - 10.1073/pnas.152155599
DO - 10.1073/pnas.152155599
M3 - Article
C2 - 12089339
AN - SCOPUS:0037047108
VL - 99
SP - 9178
EP - 9183
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 14
ER -