Transactivation induced by human T-lymphotropic virus type III (HTLV III) maps to a viral sequence encoding 58 amino acids and lacks tissue specificity

Leonard J. Seigel, Lee Ratner, Steven F. Josephs, David Dersefi, Mark B. Feinberg, Gregory R. Reyes, Stephen J. O'Brien, Flossie Wong-Staal

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79 Scopus citations

Abstract

The acquired immune deficiency syndrome (AIDS) retrovirus, HTLV-III/LAV, encodes a transacting factor which directly or indirectly stimulates the expression of genes linked to its LTR. To further dissect this phenomenon, we have cotransfected a biologically active molecular clone of HTLV-III and a recombinant plasmid containing an indicator gene, the bacterial gene for chloramphenicol acetyltransferase (CAT), under the control of the HTLV-III LTR. Amplified CAT activity was detected in both lymphoid cells and fibroblasts from a number of species in the presence of the proviral DNA. Deletion experiments confirm the previous assignment of the gene required for transactivation to a region immediately 5′ to the envelope gene, and further narrow down the critical functional domain to a coding sequence of 58 codons.

Original languageEnglish
Pages (from-to)226-231
Number of pages6
JournalVirology
Volume148
Issue number1
DOIs
StatePublished - Jan 15 1986

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