Abstract
The acquired immune deficiency syndrome (AIDS) retrovirus, HTLV-III/LAV, encodes a transacting factor which directly or indirectly stimulates the expression of genes linked to its LTR. To further dissect this phenomenon, we have cotransfected a biologically active molecular clone of HTLV-III and a recombinant plasmid containing an indicator gene, the bacterial gene for chloramphenicol acetyltransferase (CAT), under the control of the HTLV-III LTR. Amplified CAT activity was detected in both lymphoid cells and fibroblasts from a number of species in the presence of the proviral DNA. Deletion experiments confirm the previous assignment of the gene required for transactivation to a region immediately 5′ to the envelope gene, and further narrow down the critical functional domain to a coding sequence of 58 codons.
Original language | English |
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Pages (from-to) | 226-231 |
Number of pages | 6 |
Journal | Virology |
Volume | 148 |
Issue number | 1 |
DOIs | |
State | Published - Jan 15 1986 |