trans-2-Phenylcyclopropylamine is a substrate for and inactivator of horseradish peroxidase

Lawrence M. Sayre, Robert T. Naismith, Michael A. Bada, Wen Shan Li, Molly E. Klein, Mark D. Tennant

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Horseradish peroxidase (HRP) is well known for mediating the electron-transfer oxidation of electron-rich aromatic 'donors' such as phenols and anilines, but has not been described to oxidize aliphatic amines. We here confirm the inability of HRP to oxidize typical aliphatic amines, even those which would exist significantly as free bases at the operative pH. In contrast, trans-2-phenylcyclopropylamine (2-PCPA) is both a substrate (turnover product is cinnamaldehyde) and a time-dependent inactivator of HRP. These activities of 2PCPA are consistent with either a concerted or rapid sequential one-electron-oxidation/ring-opening to give an intermediate capable of covalent binding to the enzyme. 2-PCPA is the first known example of a simple aliphatic amine which serves as a substrate for HRP under turnover conditions.

Original languageEnglish
Pages (from-to)250-256
Number of pages7
JournalBiochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
Volume1296
Issue number2
DOIs
StatePublished - Sep 5 1996

Keywords

  • Amine oxidation
  • Horseradish peroxidase
  • trans-2-Phenylcyclopropylamine

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