TY - JOUR
T1 - Trafficking of Estrella lausannensis in human macrophages
AU - Rusconi, Brigida
AU - Kebbi-Beghdadi, Carole
AU - Greub, Gilbert
N1 - Funding Information:
This work was supported by the Swiss National Science Foundation (project n◦ PDFMP3–127302). Brigida Rusconi was supported by the Swiss National Science Foundation within the PRODOC program ‘Infection and Immunity’.
Funding Information:
We thank the Cellular Imaging Facility (CIF, UNIL, Switzerland) for discussion and technical support.We thank L. Pilloux (CHUV, Switzerland) for thoughtful discussions. This work was supported by the Swiss National Science Foundation (project no PDFMP3-127302). Brigida Rusconi was supported by the Swiss National Science Foundation within the PRODOC program 'Infection and Immunity'.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Estrella lausannensis is a new member of the Chlamydiales order. Like other Chlamydia-related bacteria, it is able to replicate in amoebae and in fish cell lines. A preliminary study investigating the pathogenic potential of Chlamydia-related bacteria found a correlation between antibody response to E. lausannensis and pneumonia in children. To further investigate the pathogenic potential of E. lausannensis, we determined its ability to grow in human macrophages and its intracellular trafficking. The replication in macrophages resulted in viable E. lausannensis; however, it caused a significant cytopathic effect. The intracellular trafficking of E. lausannensis was analyzed by determining the interaction of the Estrella-containing inclusions with various endocytic markers as well as host organelles. The E. lausannensis inclusion escaped the endocytic pathway rapidly avoiding maturation into phagolysosomes by preventing both EEA-1 and LAMP-1 accumulation. Compared to Waddlia chondrophila, another Chlamydia-related bacteria, the recruitment of mitochondria and endoplasmic reticulum was minimal for E. lausannensis inclusions. Estrella lausannensis appears to use a distinct source of nutrients and energy compared to other members of the Chlamydiales order. In conclusion, we hypothesize that E. lausannensis has a restricted growth in human macrophages, due to its reduced capacity to control programmed cell death.
AB - Estrella lausannensis is a new member of the Chlamydiales order. Like other Chlamydia-related bacteria, it is able to replicate in amoebae and in fish cell lines. A preliminary study investigating the pathogenic potential of Chlamydia-related bacteria found a correlation between antibody response to E. lausannensis and pneumonia in children. To further investigate the pathogenic potential of E. lausannensis, we determined its ability to grow in human macrophages and its intracellular trafficking. The replication in macrophages resulted in viable E. lausannensis; however, it caused a significant cytopathic effect. The intracellular trafficking of E. lausannensis was analyzed by determining the interaction of the Estrella-containing inclusions with various endocytic markers as well as host organelles. The E. lausannensis inclusion escaped the endocytic pathway rapidly avoiding maturation into phagolysosomes by preventing both EEA-1 and LAMP-1 accumulation. Compared to Waddlia chondrophila, another Chlamydia-related bacteria, the recruitment of mitochondria and endoplasmic reticulum was minimal for E. lausannensis inclusions. Estrella lausannensis appears to use a distinct source of nutrients and energy compared to other members of the Chlamydiales order. In conclusion, we hypothesize that E. lausannensis has a restricted growth in human macrophages, due to its reduced capacity to control programmed cell death.
KW - Chlamydia-related bacteria
KW - Cytopathic effect
KW - Golgi
KW - Intracellular bacteria
KW - Mitochondria
UR - http://www.scopus.com/inward/record.url?scp=85001833267&partnerID=8YFLogxK
U2 - 10.1093/femspd/ftv027
DO - 10.1093/femspd/ftv027
M3 - Article
C2 - 25857735
AN - SCOPUS:85001833267
SN - 2049-632X
VL - 73
JO - Pathogens and disease
JF - Pathogens and disease
IS - 5
ER -