Tracking the subcellular fate of 20(S)-Hydroxycholesterol with click chemistry reveals a transport pathway to the Golgi

Sara M. Peyrot, Sigrid Nachtergaele, Giovanni Luchetti, Laurel K. Mydock-McGrane, Hideji Fujiwara, David Scherrer, Andrew Jallouk, Paul H. Schlesinger, Daniel S. Ory, Douglas F. Covey, Rajat Rohatgi

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12 Scopus citations

Abstract

Background: Oxysterols are a class of emerging signaling molecules whose cell biology is poorly understood. Results: A click chemistry-based imaging strategy shows that 20(S)-hydroxycholesterol accumulates in Golgi membranes in a process that depends on ATP and lysosome function. Conclusion: 20(S)-Hydroxycholesterol is transported through a vesicular pathway to the Golgi. Significance: Specific transport pathways may regulate the oxysterol content of cellular membranes.

Original languageEnglish
Pages (from-to)11095-11110
Number of pages16
JournalJournal of Biological Chemistry
Volume289
Issue number16
DOIs
StatePublished - Apr 18 2014

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    Peyrot, S. M., Nachtergaele, S., Luchetti, G., Mydock-McGrane, L. K., Fujiwara, H., Scherrer, D., Jallouk, A., Schlesinger, P. H., Ory, D. S., Covey, D. F., & Rohatgi, R. (2014). Tracking the subcellular fate of 20(S)-Hydroxycholesterol with click chemistry reveals a transport pathway to the Golgi. Journal of Biological Chemistry, 289(16), 11095-11110. https://doi.org/10.1074/jbc.M113.540351