TY - JOUR
T1 - Tracking the subcellular fate of 20(S)-Hydroxycholesterol with click chemistry reveals a transport pathway to the Golgi
AU - Peyrot, Sara M.
AU - Nachtergaele, Sigrid
AU - Luchetti, Giovanni
AU - Mydock-McGrane, Laurel K.
AU - Fujiwara, Hideji
AU - Scherrer, David
AU - Jallouk, Andrew
AU - Schlesinger, Paul H.
AU - Ory, Daniel S.
AU - Covey, Douglas F.
AU - Rohatgi, Rajat
PY - 2014/4/18
Y1 - 2014/4/18
N2 - Background: Oxysterols are a class of emerging signaling molecules whose cell biology is poorly understood. Results: A click chemistry-based imaging strategy shows that 20(S)-hydroxycholesterol accumulates in Golgi membranes in a process that depends on ATP and lysosome function. Conclusion: 20(S)-Hydroxycholesterol is transported through a vesicular pathway to the Golgi. Significance: Specific transport pathways may regulate the oxysterol content of cellular membranes.
AB - Background: Oxysterols are a class of emerging signaling molecules whose cell biology is poorly understood. Results: A click chemistry-based imaging strategy shows that 20(S)-hydroxycholesterol accumulates in Golgi membranes in a process that depends on ATP and lysosome function. Conclusion: 20(S)-Hydroxycholesterol is transported through a vesicular pathway to the Golgi. Significance: Specific transport pathways may regulate the oxysterol content of cellular membranes.
UR - http://www.scopus.com/inward/record.url?scp=84898955768&partnerID=8YFLogxK
U2 - 10.1074/jbc.M113.540351
DO - 10.1074/jbc.M113.540351
M3 - Article
C2 - 24596093
AN - SCOPUS:84898955768
SN - 0021-9258
VL - 289
SP - 11095
EP - 11110
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 16
ER -