Abstract
The choroid plexus is a vital brain barrier and source of cerebrospinal fluid. Using two-photon imaging of choroid plexus epithelium in live explants and in awake mice, Shipley et al. establish a platform for investigating subcellular calcium activity and secretion in epithelial cells and diverse surveillance behaviors in immune cells.
Original language | English |
---|---|
Pages (from-to) | 623-639.e10 |
Journal | Neuron |
Volume | 108 |
Issue number | 4 |
DOIs | |
State | Published - Nov 25 2020 |
Keywords
- calcium activity
- cerebrospinal fluid
- choroid plexus
- epithelial cells
- immune cells
- secretion
- serotonin
- two-photon imaging
Fingerprint
Dive into the research topics of 'Tracking Calcium Dynamics and Immune Surveillance at the Choroid Plexus Blood-Cerebrospinal Fluid Interface'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver
}
Tracking Calcium Dynamics and Immune Surveillance at the Choroid Plexus Blood-Cerebrospinal Fluid Interface. / Shipley, Frederick B.; Dani, Neil; Xu, Huixin et al.
In: Neuron, Vol. 108, No. 4, 25.11.2020, p. 623-639.e10.Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Tracking Calcium Dynamics and Immune Surveillance at the Choroid Plexus Blood-Cerebrospinal Fluid Interface
AU - Shipley, Frederick B.
AU - Dani, Neil
AU - Xu, Huixin
AU - Deister, Christopher
AU - Cui, Jin
AU - Head, Joshua P.
AU - Sadegh, Cameron
AU - Fame, Ryann M.
AU - Shannon, Morgan L.
AU - Flores, Vanessa I.
AU - Kishkovich, Thomas
AU - Jang, Emily
AU - Klein, Eric M.
AU - Goldey, Glenn J.
AU - He, Kangmin
AU - Zhang, Yong
AU - Holtzman, Michael J.
AU - Kirchhausen, Tomas
AU - Wyart, Claire
AU - Moore, Christopher I.
AU - Andermann, Mark L.
AU - Lehtinen, Maria K.
N1 - Funding Information: We thank members of the Lehtinen, Andermann, and Moore labs for helpful discussions; A. Lutas, K. Fernando, S. Marsh, M. Webb, C. Chen, O. Alturkistani, and the IDDRC Imaging Core for advice and technical assistance; M. Bhaumik, BCH Genome Editing Core, and G. Marsischky for Htr2cmRuby3 design; M. Ericsson, HMS EM Facility; W.H. Fowle, NEU EM Facility; C. Wu at the BCH Viral Core; S. Gupton for VAMP3 expression vector; and J. Zhang for Z310 cells. This work was supported by a National Science Foundation (NSF) Graduate Research Fellowship (F.B.S.); a Glenn Foundation/American Federation for Aging Research (AFAR) Postdoctoral Fellowship and a Reagan Sloane Shanley Research Internship (N.D.); a William Randolph Hearst Fellowship (N.D. J.C.); NIH grant T32 HL110852 (J.C. R.M.F.); an Office of Faculty Development (OFD)/Basic/Translational Research Executive Committee (BTREC)/Clinical and Translational Research Executive Committee (CTREC) Faculty Development Fellowship Award (R.M.F.); NIH National Institute of Allergy and Infectious Diseases (NIAID) grant R01-AI130591 (T Kishkovich, K.H.); National Heart, Lung, and Blood Institute (NHLBI) grant R35-HL145242 (M.J.H.); GM075252 (T Kirchhausen); a gift from the Korn family and NSF Next Generation Networks for Neuroscience (NeuroNex) grant 1707352 (C.I.M.); the David Mahoney Neuroimaging Grant Program – Dana Foundation, NIH grants DP2 DK105570, DP1 AT010971, R01 DK109930, R21 EY03043-02, the McKnight Foundation, the Pew Foundation, the Smith Family Foundation, the Klarman Family Foundation, and the AFAR (M.L.A.); the Pediatric Hydrocephalus Foundation, a Tommy Fuss Center Innovation Grant, Simons Foundation Autism Research Initiative [SFARI] award 610670, a Harvard Brain Science Initiative Bipolar Disorder Seed Grant, and NIH grant R01 NS088566 (M.K.L.); Human Frontier Science Program grant RGP0063/2018 (C.W. M.K.L.); NIH grant RF1DA048790 (C.I.M. M.K.L.); New York Stem Cell Foundation (NYSCF) grants NYSCF-R-NI39 (C.W.) and NYSCF-R-NI38 (M.K.L.); and Boston Children's Hospital (BCH) Intellectual and Developmental Disabilities Research Center (IDDRC) grant 1U54HD090255. C.W. and M.K.L. are New York Stem Cell Foundation – Robertson Investigators. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. F.B.S. N.D. M.L.A. C.I.M. and M.K.L. designed the study. N.D. and F.B.S. developed whole-tissue explants and imaging protocols. C.D. and M.L.A. developed the in vivo two-photon calcium imaging protocol. F.B.S. performed all computational analyses of all imaging data with advice from M.L.A. J.C. helped generate Htr2cmRuby3 mice. F.B.S. J.C. and M.L.S. analyzed peripheral activation of immune cells. H.X. analyzed secretory pathways and developed the vesicle imaging assay with assistance from C.W. K.H. and T. Kirchhausen. J.H. developed immediate-early gene assays. G.J.G. developed and T. Kishkovich, E.J. E.K. V.I.F. and C.S. refined the imaging cannula approach. J.C. and R.M.F. analyzed CSF cytokines. Y.Z. and M.J.H. shared FoxJ1-Cre mice. F.B.S. M.L.A. and M.K.L. wrote the manuscript. The authors declare no competing interests. Funding Information: We thank members of the Lehtinen, Andermann, and Moore labs for helpful discussions; A. Lutas, K. Fernando, S. Marsh, M. Webb, C. Chen, O. Alturkistani, and the IDDRC Imaging Core for advice and technical assistance; M. Bhaumik, BCH Genome Editing Core, and G. Marsischky for Htr2c mRuby3 design; M. Ericsson, HMS EM Facility; W.H. Fowle, NEU EM Facility; C. Wu at the BCH Viral Core; S. Gupton for VAMP3 expression vector; and J. Zhang for Z310 cells. This work was supported by a National Science Foundation (NSF) Graduate Research Fellowship (F.B.S.); a Glenn Foundation /American Federation for Aging Research (AFAR) Postdoctoral Fellowship and a Reagan Sloane Shanley Research Internship (N.D.); a William Randolph Hearst Fellowship (N.D., J.C.); NIH grant T32 HL110852 (J.C., R.M.F.); an Office of Faculty Development (OFD)/Basic/Translational Research Executive Committee (BTREC)/Clinical and Translational Research Executive Committee (CTREC) Faculty Development Fellowship Award (R.M.F.); NIH National Institute of Allergy and Infectious Diseases (NIAID) grant R01-AI130591 (T Kishkovich, K.H.); National Heart, Lung, and Blood Institute (NHLBI) grant R35-HL145242 (M.J.H.); GM075252 (T Kirchhausen); a gift from the Korn family and NSF Next Generation Networks for Neuroscience (NeuroNex) grant 1707352 (C.I.M.); the David Mahoney Neuroimaging Grant Program – Dana Foundation , NIH grants DP2 DK105570 , DP1 AT010971 , R01 DK109930 , R21 EY03043-02 , the McKnight Foundation , the Pew Foundation , the Smith Family Foundation , the Klarman Family Foundation , and the AFAR (M.L.A.); the Pediatric Hydrocephalus Foundation , a Tommy Fuss Center Innovation Grant, Simons Foundation Autism Research Initiative [SFARI] award 610670 , a Harvard Brain Science Initiative Bipolar Disorder Seed Grant, and NIH grant R01 NS088566 (M.K.L.); Human Frontier Science Program grant RGP0063/2018 (C.W., M.K.L.); NIH grant RF1DA048790 (C.I.M., M.K.L.); New York Stem Cell Foundation (NYSCF) grants NYSCF-R-NI39 (C.W.) and NYSCF-R-NI38 (M.K.L.); and Boston Children's Hospital (BCH) Intellectual and Developmental Disabilities Research Center (IDDRC) grant 1U54HD090255 . C.W. and M.K.L. are New York Stem Cell Foundation – Robertson Investigators. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: © 2020 Elsevier Inc.
PY - 2020/11/25
Y1 - 2020/11/25
N2 - The choroid plexus is a vital brain barrier and source of cerebrospinal fluid. Using two-photon imaging of choroid plexus epithelium in live explants and in awake mice, Shipley et al. establish a platform for investigating subcellular calcium activity and secretion in epithelial cells and diverse surveillance behaviors in immune cells.
AB - The choroid plexus is a vital brain barrier and source of cerebrospinal fluid. Using two-photon imaging of choroid plexus epithelium in live explants and in awake mice, Shipley et al. establish a platform for investigating subcellular calcium activity and secretion in epithelial cells and diverse surveillance behaviors in immune cells.
KW - calcium activity
KW - cerebrospinal fluid
KW - choroid plexus
KW - epithelial cells
KW - immune cells
KW - secretion
KW - serotonin
KW - two-photon imaging
UR - http://www.scopus.com/inward/record.url?scp=85087440206&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2020.08.024
DO - 10.1016/j.neuron.2020.08.024
M3 - Article
C2 - 32961128
AN - SCOPUS:85087440206
SN - 0896-6273
VL - 108
SP - 623-639.e10
JO - Neuron
JF - Neuron
IS - 4
ER -