Trace driven registration of neuron confocal microscopy stacks

Luke Hogrebe, Antonio R.C. Paiva, Elizabeth Jurrus, Cameron Christensen, Michael Bridge, J. R. Korenberg, Tolga Tasdizen

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

Active research in the area of 3-D neurite tracing has predominantly focused on single sections. Ultimately, however, neurobiologists desire to study the long range connectivity of the brain, which requires tracing axons across multiple serially-cut sections. Registration of axonal sections is challenging due to several factors, such as sparseness of the axons and complications of the sectioning process, including tissue deformation and loss. This paper investigates a method for registering sections using centerline traces which provide the locations of axons at section boundaries and the angles at which the axons approach the boundaries. This information is used to determine correspondences between two serial sections. Both global and local differences are accounted for using rigid and non-rigid transforms. Results show that utilizing information from traced axons allows axon continuity across sections to be restored.

Original languageEnglish
Title of host publication2011 8th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI'11
Pages1345-1348
Number of pages4
DOIs
StatePublished - 2011
Event2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11 - Chicago, IL, United States
Duration: Mar 30 2011Apr 2 2011

Publication series

NameProceedings - International Symposium on Biomedical Imaging
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Conference

Conference2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11
Country/TerritoryUnited States
CityChicago, IL
Period03/30/1104/2/11

Keywords

  • Axonal section registration
  • Non-rigid point registration
  • Serial section registration

Fingerprint

Dive into the research topics of 'Trace driven registration of neuron confocal microscopy stacks'. Together they form a unique fingerprint.

Cite this