TY - GEN
T1 - Trace driven registration of neuron confocal microscopy stacks
AU - Hogrebe, Luke
AU - Paiva, Antonio R.C.
AU - Jurrus, Elizabeth
AU - Christensen, Cameron
AU - Bridge, Michael
AU - Korenberg, J. R.
AU - Tasdizen, Tolga
PY - 2011
Y1 - 2011
N2 - Active research in the area of 3-D neurite tracing has predominantly focused on single sections. Ultimately, however, neurobiologists desire to study the long range connectivity of the brain, which requires tracing axons across multiple serially-cut sections. Registration of axonal sections is challenging due to several factors, such as sparseness of the axons and complications of the sectioning process, including tissue deformation and loss. This paper investigates a method for registering sections using centerline traces which provide the locations of axons at section boundaries and the angles at which the axons approach the boundaries. This information is used to determine correspondences between two serial sections. Both global and local differences are accounted for using rigid and non-rigid transforms. Results show that utilizing information from traced axons allows axon continuity across sections to be restored.
AB - Active research in the area of 3-D neurite tracing has predominantly focused on single sections. Ultimately, however, neurobiologists desire to study the long range connectivity of the brain, which requires tracing axons across multiple serially-cut sections. Registration of axonal sections is challenging due to several factors, such as sparseness of the axons and complications of the sectioning process, including tissue deformation and loss. This paper investigates a method for registering sections using centerline traces which provide the locations of axons at section boundaries and the angles at which the axons approach the boundaries. This information is used to determine correspondences between two serial sections. Both global and local differences are accounted for using rigid and non-rigid transforms. Results show that utilizing information from traced axons allows axon continuity across sections to be restored.
KW - Axonal section registration
KW - Non-rigid point registration
KW - Serial section registration
UR - http://www.scopus.com/inward/record.url?scp=80055037594&partnerID=8YFLogxK
U2 - 10.1109/ISBI.2011.5872649
DO - 10.1109/ISBI.2011.5872649
M3 - Conference contribution
AN - SCOPUS:80055037594
SN - 9781424441280
T3 - Proceedings - International Symposium on Biomedical Imaging
SP - 1345
EP - 1348
BT - 2011 8th IEEE International Symposium on Biomedical Imaging
T2 - 2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11
Y2 - 30 March 2011 through 2 April 2011
ER -