Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite

J. M. Dobrowolski, L. D. Sibley

Research output: Contribution to journalArticlepeer-review

503 Scopus citations

Abstract

Toxoplasma gondii is an obligate intracellular parasite that invades a wide range of vertebrate host cells. We demonstrate that invasion is critically dependent on actin filaments in the parasite, but not the host cell. Invasion into cytochalasin D (CD)-resistant host cells was blocked by CD, while parasite mutants invaded wild-type host cells in the presence of drug. CD resistance in Toxoplasma was mediated by a point mutation in the single-copy actin gene ACT1. Transfection of the mutant act1 allele into wild-type Toxoplasma conferred motility and invasion in the presence of CD. We conclude that host cell invasion by Toxoplasma, and likely by related Apicomplexans, is actively powered by an actin-based contractile system in the parasite.

Original languageEnglish
Pages (from-to)933-939
Number of pages7
JournalCell
Volume84
Issue number6
DOIs
StatePublished - Jan 1 1996

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